粘液型铜绿假单胞菌藻酸盐对免疫细胞功能影响  

Effect of Mucoid Pseudomonas aeruginosa Alginate on Immunocyte

作　　者：王睿[1] 柴栋[1] 裴斐[1] 方翼[1] 朱曼[1] 王中孝[1] 

机构地区：[1]解放军总医院,北京100853

出　　处：《中华医院感染学杂志》2004年第1期25-27,共3页Chinese Journal of Nosocomiology

基　　金：国家自然科学基金课题 (30 0 70 72 0 )

摘　　要：目的　探讨铜绿假单胞菌生物被膜感染免疫逃逸机制。方法　以琼脂糖平板法、鲁米诺发光试验以及MTT比色法分别测定中性粒细胞、淋巴细胞趋化、吞噬以及转化功能 ,考察铜绿假单胞菌藻酸盐对免疫细胞功能的影响。结果　低浓度铜绿假单胞菌藻酸盐 (5 μg/ m l)对中性粒细胞的趋化以及吞噬功能 ,以及淋巴细胞的转化功能的抑制率分别为 10 .5 7%、2 2 .33%、2 5 .70 %。结论　铜绿假单胞菌生物被膜感染免疫逃逸可能与其分泌藻酸盐免疫抑制作用密切相关。OBJECTIVETo investigate the mechanisms involved in the evasion of host defense by Pseudomonas aeruginosa biofilms. METHODSThe functions of neutrophil and lymphocyte were determined by locomotion test, chemiluminescence test and lymphocyte transformation test. RESULTS Neutrophil locomotion, chemiluminescence and lymphocyte transformation were significantly reduced by 5 μg/ml P. aeruginosa alginate by 10.57%, 22.33% and 25.70%. CONCLUSIONS The inhibition of P. aeruginosa alginate on immunocyte is connected closely with the evasion of host defense by P. aeruginosa biofilms.

关 键 词：铜绿假单胞菌藻酸盐 免疫细胞 细菌生物被膜 

分 类 号：R378.991[医药卫生—病原生物学]