快速原位诊断中心静脉导管相关性血行感染方法的建立  

Central-venous-catheter-related Bloodstream Infection:An in situ Method for Rapid Diagnosis

作　　者：杨燕妮[1] 邹英[1] 伍素华[1] 张杰[1] 王殿珍[1] 段敏[1] 钟华[1] 

机构地区：[1]第三军医大学西南医院,重庆400038

出　　处：《中华医院感染学杂志》2004年第1期113-115,共3页Chinese Journal of Nosocomiology

摘　　要：目的　建立并评价一种快速原位诊断中心静脉导管相关性血行感染 (CRBSI)的方法。方法　应用革兰 -吖啶橙白细胞染色检测法检测 115例疑为中心静脉 CRBSI的患者 ,与常规培养方法相比较 ,评价其敏感性、特异性及可行性。结果　 115例中有 4 3例 (37% )被革兰 -吖啶橙白细胞染色检测法诊断为中心静脉 CRBSI,以常规方法作参照 ,敏感性、特异性、阳性预测值、阴性预测值及准确度分别为 95 %、93%、88%、97%、94 % ,在对革兰阳性菌、革兰阴性菌和真菌鉴别诊断中 ,亦获得了较高的敏感性和特异性。结论　革兰 -吖啶橙白细胞染色检测法可作为原位诊断中心静脉 CRBSI的简单、实用的方法 ,有助于早期诊断及指导针对性应用抗生素。OBJECTIVETo establish and evaluate an in situ method for the rapid diagnosis of central-venous-catheter-related bloodstream infection (CRBSI). METHODS The Gram-acridine-orange leucocyte stain test was used in 115 suspected cases of CRBSI, and its sensitivity, specificity and feasibility were compared with routine cultural method. RESULTSForty three cases (37%) were diagnosed as CRBSI by the Gram-acridine-orange leucocyte stain test. The sensitivity of the test was 95%, while 93%, 94%, 88%, 97% for the specificity, accuracy, positive and negative predictive value, respectively. And it also had relatively high sensitivity and specificity on the identification of G+, G-bacteria and fungi. CONCLUSIONS The Gram-acridine-orange leucocyte stain test is a simple, quick, economical and readily available method in the early diagnosis of CRBSI without catheter removal, and may facilitate treatment with early targeted antimicrobials.

关 键 词：中心静脉导管 感染 诊断 

分 类 号：R473[医药卫生—护理学]