非4℃条件储运后CPDA血液保存期的变化  

The Changes of CPDA Whold Blood's Shelf Life after Stored at Non-4℃

作　　者：刘景汉[1] 韩玮[1] 潘纪春[1] 于洋[1] 庄远[1] 

机构地区：[1]解放军总医院输血科

出　　处：《中国医师杂志》2004年第11期1482-1483,共2页Journal of Chinese Physician

基　　金："十五"军队医药卫生科研基金指令性课题资助项目 (0 1L0 4 5)

摘　　要：目的　为研究非 4℃条件储运不同时间后CPDA血液保存有效期的变化。方法　采集 10名健康献血者全血各 2 0 0ml ,每袋分出 5 0ml标记为对照组 ,其余标记为实验组 ;将对照组置于 4℃冰箱保存 ,于保存末期检测红细胞ATP含量为临界ATP ;将实验组置于 10℃保存 2 4h ,然后放入 4℃冰箱继续保存 ,每天测定ATP等指标 ,以临界ATP为指示点确定剩余保存有效期。重复上述方法 ,测定血液在不同温度保存 48h和 72h后的剩余保存有效期。结果　血液常温储存 2 4h保存有效期变化不明显 ,储存 48h以上则保存有效期明显缩短 ,温度越高剩余保存有效期越短。结论　血液在常温下储运 2 4h～ 72h ,入库 ( 4℃冰箱 )后应相应确定继续保存时间 ,以保证血液保存质量和输注疗效。Objective To study the changes of CPDA whole blood's shelf life after stored at non-4℃. Methods 200 ml whole blood was collected from each of 10 donors and anticoagulated by CPDA. Then 50 ml whole blood from each one was marked as control group, the rest was marked as test group. The blood of the control group was stored at 4℃ and RBC ATP concentration at the end of its shelf life served as critical ATP. The blood of the test group was stored at 10℃ for 24 hours, then it was transferred to 4℃ refrigeratory and continued to preserve. RBC ATP concentration were tested daily to ensure it eligible. When RBC ATP concentration decreased to the level of critical ATP, the time of preservation at 4℃ was blood's succeeding shelf life. Using the same method, the succeeding shelf life of CPDA whole blood after stored at different temperature for 48 to 72 hours was measured. Results The succeeding shelf life of CPDA whole blood did not obviously change after stored at non-4℃ for 24 hours, while it shortened dramaticly after stored at non-4℃ for more than 48 hours. The higher the temperature, the shorter the succeeding shelf life. Conclusion The results suggested that CPDA whole blood's succeeding shelf life should be adjusted after stored at non-4℃ for 24 to 72 hours to ensure the blood quality.

关 键 词：血液保存 有效期 对照组 ATP CPDA 不同时间 输注 条件 结论 变化 

分 类 号：R197.3[医药卫生—卫生事业管理]