人在位和异位子宫内膜腺上皮细胞和间质细胞的原代培养  

Primary culture of human eutopic and ectopic endometrial glandular epithelial cells and stromal cells

作　　者：石书芳[1] 俞超芹[1] 刘玉环[2] 崔英[2] 惠宁[2] 

机构地区：[1]第二军医大学长海医院中医科,上海200433 [2]长海医院妇产科

出　　处：《第二军医大学学报》2004年第11期1228-1231,共4页Academic Journal of Second Military Medical University

基　　金：国家自然科学基金 ( 3 0 0 70 942 )

摘　　要：目的 :探讨在位和异位子宫内膜腺上皮细胞和间质细胞分离培养方法 ,为研究子宫内膜异位症的发病机制提供体外细胞模型。方法 :通过消化、过滤、沉降等方法 ,分离培养正常对照子宫内膜以及子宫内膜异位症在位、异位子宫内膜腺上皮细胞和间质细胞 ,模拟人体内雌激素水平研究促进细胞生长的方法 ,光学显微镜观察离体细胞形态。结果 :正常对照子宫内膜细胞分离培养成功率达 91.7% (11/ 12 ) ,子宫内膜异位症在位子宫内膜细胞成功率为 93.8% (15 / 16 ) ,子宫内膜异位症异位子宫内膜细胞成功率为 75 .0 % (12 / 16 )。间质细胞传代 2次后 ,生长缓慢 ,经雌激素作用后 ,生长明显改善 ;腺上皮细胞不能传代 ,经雌激素作用后 ,生长改善不明显。结论 :体外分离培养的人子宫内膜细胞比子宫内膜异位动物模型更接近于人体的特点 ,在位和异位子宫内膜腺上皮细胞和间质细胞分离培养可作为研究子宫内膜异位症的体外细胞模型之一。Objective:To explore the way to separate and culture eutopic and ectopic endometrial glandular cells and their stromal cells,providing an in vitro cell model of endometriosis for study of its mechanism. Methods:Digestion,filtration and sedimentation were used to isolate and culture the eutopic and ectopic endometrial glandular cells and their stromal cells. The estrogen level was imatated to study the way of promoting cell growth. Morphological characters of eutopic and ectopic endometrial cells were examined using optical microscope. Results:The success rate of separation and culture of normal control endometrial glandular cells and its stromal cells was 91.7%(11/12);of eutopic endometrial glandular cells and its stromal cells of endometriosis was 93.8%(15/16);of etopic endometrial glandular cells and its stromal cells of endometriosis was 75.0%(12/16) . Conclusion:The cultured eutopic and ectopic endometrial cells is more like human body feature than the endometriosis model of animals. So the isolation and culture of eutopic and ectopic endometrial glandular cells and their stromal cells may serve as an in vitro experimental model.

关 键 词：子宫内膜异位症 子宫内膜 腺上皮细胞 间质细胞 细胞培养 

分 类 号：R711.71[医药卫生—妇产科学]