SARS康复者及医护人员冠状病毒的初步调查  

A preliminary report of SARS coronavirus specific RNA in SARS convalescents and medical person

作　　者：贾兴旺[1] 田亚平[1] 沈洪[2] 宋淑珍[1] 袁彬[2] 邓新心[1] 

机构地区：[1]解放军总医院生化科,北京100853 [2]解放军总医院急诊科

出　　处：《中国危重病急救医学》2004年第11期667-669,共3页Chinese Critical Care Medicine

基　　金：解放军总医院 SARS防治专项研究 (2 0 0 3 SARS11;2 0 0 3 SARS0 3 )

摘　　要：目的　采用荧光聚合酶链反应 (F PCR)和基因芯片技术检测严重急性呼吸综合征 (SARS)冠状病毒 ,并探讨其临床应用价值。方法　应用 SARS Co V F PCR诊断试剂盒及基因芯片技术检测了 6 0份确诊 SARS患者血清、发热门诊医护人员血清 2 0份和漱口液样本 2 0份以及 1份 SARS疑似患者血清的 c DNA。结果　中山大学达安基因股份有限公司及上海复星实业股份有限公司的两种 F PCR检测试剂盒及晶宇芯片反应 3种检测方法所测 80份血清和 2 0份痰液样本均为阴性 ;但 1例 SARS疑似患者血清的 c DNA可经荧光定量 PCR反应扩增出病毒特异 RNA片段。结论　 SARS康复者及密切接触医护人员血液和漱口水中均未检测到 SARS病毒特异 RNA片段。Objective To observe the method of fluorescentpolymerase chain reaction (FPCR) and gene chip technique in detecting coronavirus in severe acute respiratory syndrome (SARS) and its value for clinical application. Methods Serum of 60 SARS patients, 20 samples of serum and 20 samples of gargling fluid of medical staff of the “Fever Clinic”, and one cDNA specimen obtained from one SARS suspect patient were examined with FPCR diagnosis kit and genechip technique for SARS coronavirus. Results Three methods, including DR Chip and two FPCR kit from Zhongshan College Da'an gene stock company and Shanghai Fortune industrial jointstock Co,Ltd, were used, and the results were all negative for all specimens, except one cDNA speciment, which was obtained from one SARS suspect patient, virus could be amplified by FPCR. Conclusion Special RNA fragment of SARS virus has not been detected in gargling fluid and serum of SARS convalescents and medical staff.

关 键 词：严重急性呼吸综合征 冠状病毒 人 聚合酶链反应 漱口液 

分 类 号：R511.9[医药卫生—内科学]