铂修饰超细纤维聚苯胺在葡萄糖生物传感方面的应用  

The Application of Platinum Modified Superfine Fibrous Polyaniline in Glucose Biosensors

作　　者：周海晖[1] 陈宏[1] 宁晓辉[1] 罗胜联[2] 魏万之[2] 旷亚非[1] 

机构地区：[1]湖南大学化学化工学院,长沙410082 [2]化学生物传感与化学计量学国家重点实验室,长沙410082

出　　处：《分析化学》2004年第11期1445-1449,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金 (No .5 0 473 0 2 2 );湖南大学化学生物传感;计量学国家重点实验室基金 (No .2 0 0 2 0 1)资助项目

摘　　要：在不锈钢电极 (SS)表面制得超细纤维状聚苯胺 (superfine fibrousPANI) ,经Pt微粒修饰后得到Pt微粒超细纤维聚苯胺复合电极 [Pt/ (superfine fibrousPANI) /SS]。结果表明 ,直径 5 0～ 10 0nm的Pt微粒均匀分布于直径约 10 0nm的聚苯胺纤维上 ;Pt/ (superfine fibrousPANI) /SS电极对H2 O2 氧化具有很好的电催化活性。采用脉冲电流法 (PGM )再将葡萄糖氧化酶 (GOD)与间苯二胺 (MPD)混合共聚嵌于Pt/ (superfine fibrousPANI) /SS电极表面 ,获得了具有优异生物电化学传感特性的葡萄糖氧化酶电极。该酶电极最大响应电流密度im=917.4 μA/cm2 ,米氏常数Km=9.339mmol/L ;酶电极对葡萄糖响应快 ,对尿酸和抗坏血酸有很好的抗干扰性能。Superfine-fibrous polyaniline (PANI) was synthesized on stainless steel electrode ( SS) and superfine-fibrous PANI films modified with highly dispersed platinum microparticles [Pt/(superfine-fibrous PANI)/SS] were studied in the electrocatalytic oxidation of hydrogen peroxide. The results showed that PANI films have superfine-fibrous microstructure and Pt microparticles with a diameter of 50 similar to 100nin dispersed homogeneously on PANI superfine-fibers. The Pt/(superfine-fibrous PANI)/SS electrode has excellent electrocatalytic activity for H2O2 oxidation. In addition, a glucose biosensor based on the detection of hydrogen peroxide was prepared by electropolymerization of m-phenylenediamine onto the Pt-dispersed superfine-fibrous PANI film in the presence of glucose oxidase. The enzyme electrode has excellent performance with a very large steady-state current density of 917.4 muA/ cm(2) and the Michaelis-Menten constant of 9.339 mmol/L. A fast amperometric response time to glucose and good anti-interference to uric acid and ascorbic acid have also been observed.

关 键 词：不锈钢电极 超细纤维状聚苯胺 脉冲电流法 葡萄糖氧化酶 间苯二胺 生物传感 

分 类 号：O657.1[理学—分析化学]