Wistar大鼠2型糖尿病动物模型的建立  

Establishment of type 2 diabetic animal model in Wistar rats

作　　者：艾静[1] 王宁[1] 杜杰[1] 杨梅[1] 刘萍[1] 杜智敏[2] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学药理学教研室.黑龙江省生物医药重点实验室,黑龙江哈尔滨150086 [2]哈尔滨医科大学临床药理基地,黑龙江哈尔滨150086

出　　处：《中国药理学通报》2004年第11期1309-1312,共4页Chinese Pharmacological Bulletin

基　　金：黑龙江省科技厅重大项目基金资助项目 ;No 2 0 0 10 10 10 0 1-0 0 ;黑龙江省教育厅课题资助项目;No 10 5 3 10 94;国家自然科学基金资助项目;No 3 0 3 7164 7

摘　　要：目的　建立 2型糖尿病的动物模型。方法　①给Wistar大鼠灌胃脂肪乳 10d ,记录饮食、饮水、体重变化 ,用毛细血管法和正糖钳技术判定胰岛素抗性。同时测定血清中空腹血糖、丙二醛 (MDA)和游离脂肪酸 (FFA) ;②采用不同的给药方式给灌胃脂肪乳大鼠腹腔注射四氧嘧啶 ,将 72h后血糖值≥ 16 7mmol·L-1者定为 2型糖尿病大鼠。结果　①与正常对照组比较 ,灌胃脂肪乳大鼠的体重、饮食、饮水及内脏脂肪重量均明显增高 (P <0 0 5 ) ;②灌胃脂肪乳后 ,大鼠KITT值由正常对照组 6 8± 1 5下降为 4 5± 0 9(P <0 0 5 )。正糖钳实验结果表明高脂组大鼠葡萄糖输注速率(GIR)较正常大鼠葡萄糖输注速率明显下降 ;③四氧嘧啶给药剂量为第一天 12 0mg·kg-1,第二天 10 0mg·kg-1时 ,糖尿病动物模型成模率可达 90 %。结论　通过灌胃脂肪乳建立了伴有胰岛素抵抗的Wistar大鼠 2型糖尿病的动物模型。Aim To establish type 2 diabetes mellitus animal model with characteristic of short time, economy and simplicity. Methods ①After Wistar rats were administrated with high fat emulsion 10 d, we recorded the changes of the diets, drinking and body weight every day, assessed the insulin resistance by using hyperinsulinemic-euglycemic clamp technique and blood capillary method, blood serum fasting blood glucose, free fatty acid(FFA) and malondialdehydeto(MDA).②We injected alloxan to abdominal cavity of Wistar rats that were administrated with high fat emulsion by using different motheds. The type 2 diabetic animals were then affirmed when the blood glucose level more than 16.7 mmol·L -1 after injected alloxan 72 h. Results ①The body weight, diets, drink and visceral fat weight were marked increased compare with control group(P<0.05);②After administrated with high fat emulsion,the K ITT of Wistar rats decreased from 6.8±1.5 in normal control to 4.5±0.9(P<0.05), the GIR was also definitely descent than normal control by using hyperinsulinemic-euglycemic clamp technique(P<0.05);③The success rate of type 2 diabetes mellitus animal model arrive to 90% when the dosage of alloxan is 120/100 mg·kg -1. Conclusion Establishment a sort of type 2 diabetes mellitus animal model in Wistar rats by ig’d fat emulsion.

关 键 词：胰岛素抵抗 2型糖尿病 动物模型 四氧嘧啶 

分 类 号：R587.1[医药卫生—内分泌] R363-332[医药卫生—内科学]