结核分枝杆菌Ag85B与IL-12基因真核共表达质粒的构建及表达  

Construction and expression of the eukaryotic coexpression plasmid containing Ag85B gene of Mycobacterium tuberculosis and IL-12 gene

作　　者：江山[1] 朱道银[1] 骆旭东[1] 陈全[1] 蒋英[1] 

机构地区：[1]重庆医科大学微生物学教研室,重庆400016

出　　处：《细胞与分子免疫学杂志》2004年第6期664-666,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：重庆市卫生局重点项目资助 (No.0 0 1 0 0 6)

摘　　要：目的 :构建结核分枝杆菌Ag85B和鼠IL 12基因的共表达载体pBud85B IL12。方法 :将结核分枝杆菌Ag85B基因和鼠IL 12基因同时克隆入含多启动子的共表达载体pBudCE4 .1中 ,构建真核共表达质粒pBud85B IL12。以pBud85B IL12转染COS 7细胞 ,通过RT PCR及ELISA方法检测目的基因的表达。结果 :在COS 7细胞中同时可检测到Ag85B和IL12的表达。结论 :pBud85B IL12共表达质粒的成功构建 ,为对其免疫原性。AIM: To construct a eukaryotic coexpression plasmid containing Mycobacterium tuberculosis (MTB) Ag85B and IL-12 genes. METHODS: MTB Ag85B gene and IL-12 gene were cloned into pBudCE4.1 which has multiple promoters to construct recombinant plasmid pBud85B-IL12. The recombinant plasmid was transfected into COS-7 cells and the expression of target genes was assessed by RT-PCR and ELISA. (RESULTS:) The expressions of Ag85B and IL-12 could be detected in COS-7 cells. CONCLUSION: The recombinant plasmid pBud85B-IL12 was constructed and expressed successfully, which lays the foundation for further development of DNA vaccine against tuberculosis.

关 键 词：结核分枝杆菌 AG85B IL-12 共表达载体 

分 类 号：R378.91[医药卫生—病原生物学]