缺氧对心肌细胞内游离钙离子浓度和SERCA2表达的影响及薯蓣皂甙的干预作用  

The effect of anoxic injury and the dioscornin intervention on intracellular calcium concentration and SERCA2 expression of myocardial cells

作　　者：刘遂心[1] 孙明[1] 李彤[1] 

机构地区：[1]中南大学湘雅医院心血管病康复中心,长沙410008

出　　处：《中华心血管病杂志》2004年第9期841-843,共3页Chinese Journal of Cardiology

摘　　要：目的　研究缺氧对心肌细胞内游离钙离子浓度和肌浆网上的钙离子ATP酶(SERCA2 )的表达的影响及薯蓣皂甙的干预作用。方法　在离体心肌细胞培养基础上制备心肌细胞缺氧模型 ,随机分为 4组 :正常对照组 ,缺氧损伤组 ,薯蓣皂甙组 ,钙拮抗剂组。分别测定各组心肌细胞内游离钙离子的浓度 (FURA2 /AM荧光探针法 )和SERCA2的含量 (Westernblot法 )。结果　缺氧损伤组心肌细胞内游离钙离子的浓度较正常对照组明显增加 ,SERCA2的表达明显减弱 (P均 <0 0 1 ) ;薯蓣皂甙组和钙拮抗剂组心肌细胞内游离钙离子的浓度较缺氧损伤组明显降低 (P <0 0 1 ) ,SERCA2的表达明显增强 (P <0 0 5 )。结论　薯蓣皂甙通过增强肌浆网上钙泵SERCA2的表达减轻缺氧心肌细胞的钙超负荷 ,它对缺氧心肌细胞具有保护作用 ,其作用与钙拮抗剂类似。Objective To study the effect of the anoxic injury on dissociative calcium concentration of myocardial cells and the protective effect of the dioscornin on the calcium overload. Methods Rat myocardial cells were cultured and divided into 4 groups randomly. Then the anoxic models were set up. After that, we measured the dissociative calcium concentration of myocardial cells with fura2/am fluorescent probe and the content of the sarco/endoplasmic reticulum Ca 2+ ATPases (SERCAs), with Western blot method in each group. Results The dissociative calcium concentration of the anoxic group is much higher than that of the control group (P<0.01). However, the expression of the SERCA2 in the anoxic myocardial cells is lower than that of normal cells (P<0.01). Both dioscornin and levoanlodipine can lessen the calcium overload of cells and enhance the SERCA2 expression of the anoxic myocardial cells (P<0.05). Conclusion Dioscornin can protect the anoxic myocardial cells by attenuating the calcium overload and the mechanism is similar to that of the calcium channel blocker.

关 键 词：胞内游离钙 心肌细胞 表达 缺氧损伤 钙拮抗剂 干预作用 钙离子浓度 薯蓣皂甙 肌浆网 BLOT法 

分 类 号：R541.4[医药卫生—心血管疾病]