原代海马神经元兴奋毒性模型的建立及存活率的检测与意义  

Development of excitotoxicity model with primary hippocampal neurons and implications of viability essay

作　　者：沈洁[1] 孙长凯[2] 范明[3] 马辉[2] 林平 佟玉英[2] 施广霞[1] 

机构地区：[1]大连医科大学病理生理教研室,116027 [2]大连医科大学脑疾病研究所 [3]军事医学科学院基础医学研究所 [4]解放军第一疗养院药剂科

出　　处：《中华检验医学杂志》2004年第10期687-689,共3页Chinese Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目 ( 3 0 0 70 2 67) ;中国博士后科学基金资助项目 ( 2 0 0 1) ;辽宁省重点科技攻关课题资助项目( 2 0 0 12 2 60 0 5 )

摘　　要：目的　探讨和观察药物对神经元的损伤及保护作用。方法　在体外进行海马神经元原代培养并建立谷氨酸兴奋毒作用模型 ;采用台盼蓝染色法、乳酸脱氢酶检测和程序性细胞死检测技术进行原代培养海马神经元存活率检测。结果　神经元的损伤程度与谷氨酸浓度相关 ,NMDA受体拮抗剂MK 80 1对谷氨酸引起神经元死亡有明显的保护作用。结论　谷氨酸通过NMDA受体引起细胞损伤 。Objective To study the injury and protective action of drugs on neurons, the model of glutamate excitotoxicity on primary cultured hippocampual neurons from new born rats were( set up. Methods)By use of trypan blue dye staning and testing the lactate dehydrogenase leakage from cultured neurons, to investigate the neuron survival rate. Results We found the injury of neurons was related with the concentration of glutamate. NMDAR non-competitive antagonist —MK-801 could protect the glutamate excitotoxic damage on neurons. Conclusion The glutamate results in neuron injury through NMDAR; the model of neuron culture was sufficient for glutamate-induced excitotoxicity.

关 键 词：海马神经元 谷氨酸 兴奋毒性 毒作用 保护作用 存活率 原代培养 台盼蓝染色法 细胞 酸浓度 

分 类 号：R96[医药卫生—药理学]