骨髓间充质干细胞参与皮脂腺导管构成的初步研究  

Study on the potentiation of bone marrow mesenchymal stem cells involved in sebaceous duct formation

作　　者：方利君[1] 付小兵[1] 程飚[1] 孙同柱[1] 李建福[1] 曹荣[2] 王玉新[3] 

机构地区：[1]解放军第三○四医院全军创伤修复重点实验室 [2]第四军医大学神经生物研究所 [3]中国医科大学第一医院口腔颌面外科

出　　处：《中华外科杂志》2004年第18期1136-1138,共3页Chinese Journal of Surgery

基　　金：国家重大基础研究规划资助项目 (G19990 5 42 0 4) ;国家自然科学基金面上项目 (3 0 170 966) ;国家自然科学基金重点项目(3 0 2 3 0 3 70 ) ;国家杰出青年基金 (3 95 2 5 0 2 4)

摘　　要：目的　探讨骨髓间充质干细胞 (MSCs)分化为皮肤附属器的可行性。方法　抽取小型香猪的骨髓 ,经密度梯度离心分离、体外纯化间充质干细胞及培养扩增后 ,应用 5 溴脱氧尿嘧啶(BrdU)标记技术进行细胞标记。将已标记的细胞 (1× 10 6)以注射方式回植到提供骨髓的猪的皮内及皮下 ,分别于注射后 1、2、4周取材 ,常规石蜡包埋、切片 ,行HE染色及BrdU和角蛋白免疫荧光染色 ,激光共聚焦显微镜观察。结果　部分BrdU阳性标记细胞出现在皮脂腺周围的导管中参与皮脂腺导管的形成 ,并同时表达角蛋白。结论　在皮肤微环境下骨髓间充质干细胞可以分化为皮脂腺导管细胞。Objective To investigate the feasibility of differentiation of bone marrow mesenchymal stem cells (MSCs) into skin appandence. Methods Porcine MSCs were isolated from porcine marrow and grown in vitro. After labeling with BrdU, MSCs were engrafted to porcine skin. At 1, 2, 4 weeks after the transplantation, immunohistochemical examinations were carried out to detect the positive staining of BrdU and cytokeratin. Results A few sebaceous duct cells, which expressed cytokeratin, were also BrdU positive, and these cells were considered may to be transplanted MSCs-derived cells. Conclusion Porcine MSCs mighthave the potential to differentiated into sebaceous duct cells in skin.

关 键 词：皮脂腺 骨髓间充质干细胞 导管 角蛋白 皮肤 免疫荧光染色 回植 密度梯度离心 细胞标记 标记技术 

分 类 号：R641[医药卫生—外科学]