3个山羊群体中4个微卫星DNA多态性及其与杂种优势的关系  

Genetic polymorphism of 4 microsatellites DNA in 3 goat populations and relationship with heterosis

作　　者：张英杰[1] 赵有璋[1] 刘月琴[2] 李玉[3] 孙少华[3] 孙洪新[3] 

机构地区：[1]甘肃农业大学动物科技学院,兰州730000 [2]河北省畜牧兽医研究所,保定071000 [3]河北农业大学动物科技学院,保定071000

出　　处：《遗传》2004年第5期631-636,共6页Hereditas（Beijing)

基　　金：国家科技部"十五"攻关计划(编号:2002BA514A 4 1);河北省"十五"攻关计划(编号:01220407D)~~

摘　　要：利用4个微卫星标记(OarFCB11,OarAE101,McM218,McM38)对波尔山羊、太行山羊和河北奶山羊的等位基因频率、群体多态信息含量、有效等位基因数、杂合度和遗传距离进行了遗传检测,并测定了波尔山羊与河北奶山羊及太行山羊的杂交效果。结果表明:4个微卫星标记在波尔山羊、太行山羊和河北奶山羊3个品种中存在多态性,可以用于山羊遗传多样性的评估;从不同品种来看,太行山羊的遗传变异程度最大,而波尔山羊的遗传变异程度相对较小;波尔山羊与河北奶山羊的遗传距离大于与太行山羊,波尔山羊与河北奶山羊的杂种优势高于与太行山羊,与实际杂种优势测定结果相符。Gene frequency,polymorphism information contents,number of effective alleles,heterozygosity and genetic distances were studied in Boer goat,Taihang goat and Hebei dairy goat using four microsatellite markers(OarFCB11,OarAE101,McM218,McM38).The crossing effects on Hebei dairy goat and Taihang goat with Boer goat were tested. The results indicated that there are genetic polymorphisms at four microsatellite markers in three goat breeds. Four microsatellite markers can be used for genetic diversity evaluation in goat breeds. The genetic variability of Taihang goat is the highest,and Boer goat is the lowest in three goat breeds. Genetic distances between Boer goat and Hebei dairy goat is bigger than that between Boer goat and Taihang goat. The heterosis between Boer goat and Hebei dairy goat is higher than that between Boer goat and Taihang goat. It accords with testing results on actual heterosis.

关 键 词：山羊 微卫星DNA 遗传多态性 杂种优势 

分 类 号：S827.2[农业科学—畜牧学]