脂蛋白(a)对体外培养的人系膜细胞增殖、移行、黏附的影响  

Effect of Lp(a) on human mesangial cell proliferation, adhesion and migration

作　　者：许可[1] 宋红梅[1] 魏珉[1] 

机构地区：[1]中国医学科学院中国协和医科大学北京协和医院儿科,100730

出　　处：《中华儿科杂志》2004年第10期734-736,i002,共4页Chinese Journal of Pediatrics

摘　　要：目的　观察不同浓度脂蛋白 (a) [Lp(a) ]对体外培养的人系膜细胞 (HMC)增殖、黏附和移行的影响。以研究Lp(a)在肾损伤时HMC异常增殖过程中所起的作用。 方法　用 5 μg、10 μg、2 5μg、5 0 μg /ml浓度的Lp(a)刺激HMC增殖 2 4h ,采用3 H TdR掺入法检测Lp(a)对HMC增殖的影响。用间接免疫荧光法检测Lp(a)对HMC上黏附蛋白表达的影响。以倒置相差显微镜观察Lp(a)对HMC移行性的影响。结果　随着Lp(a)浓度的升高 ,3 H TdR掺入值 (× 10 3 cpm)分别为 :1 6 9±0 4 8、3 5 9± 0 6 8、4 14± 0 78、4 0 5± 0 5 5 ,对照组 1 6 4± 0 31,Lp(a)浓度达 10 μg时与对照组比较差异有显著意义 (P <0 0 1)。以 10 μg/mlLp(a)刺激HMC 2 4h后 ,HMC上黏附蛋白表达阳性 ,对照组为阴性。Lp(a)浓度为 10 μg /ml刺激HMC 72h可显著引起HMC的移行 (实验组 16 2个 /低倍视野 ,对照组 2 4个 /低倍视野 ,P <0 0 1)。结论　Lp(a)可对HMC的增殖、黏附和移行产生显著影响。Objective The renal disease i s commonly associated with hyperlipidemia and correlates with glomerular accumul ation of atherogenic lipoproteins and mesangial hypercellularity. Therefore, in this study, the authors investigated a possible growth stimulatory effect and m ode of action of lipoprotein(a) in human mesangial cells HMC, and the e ffect of Lp(a) on adhesion and migration in human mesangial cells. M ethods The DNA synthesis of HMC was measured by3H- thymidine incorporation. The cell adhesion was detected by the expression of vinculin by means of indirect immunofluorescence. The cell migration was observed under th e microscope. Results The incubation of HMC with Lp(a) f or 24 hours induced a significant dose-dependent proliferation of HMC [Lp(a) :5 μg, 10 μg, 25 μg, 50 μg/ml vs. control 0 μg/ml;3H-TdR incorpor ation (×103cpm):1.69 ±0.48, 3.59 ±0.68, 4.14 ±0.78, 4.05 ±0.55 vs. 1.64 ±0.31, P<0.01]. The vinculin staining by indirect i mmunofluorescence showed positive result when HMC was incubated with 10 μg /ml Lp(a) for 24 hours, while vinculin was negative when HMC was incubated with 0 μg/ml Lp(a) as the control of the study. The incubation of HMC with 10 μg / ml Lp(a) for 72 hours demonstrated significant cell migration effect compared to the control of 0 μg /ml.(16.2/LP vs. 2.4/LP, P<0.01). Conclusion Lp(a) could stimulate a proliferation, adhesion and migration effect on human mesangial cells.

关 键 词：Lp(a) 对照组 增殖 移行 黏附 脂蛋白(a) 影响 浓度 显微镜观察 体外培养 

分 类 号：R692[医药卫生—泌尿科学]