早期不同饮食营养干预对宫内生长迟缓幼鼠生长追赶的影响  

Effect of different early nutritional interventions on catch-up growth of rats with intrauterine growth retardation

作　　者：丘小汕[1] 黄婷婷[1] 沈振宇[1] 邓会英[2] 柯志勇[1] 梅开勇[3] 赖峰[1] 

机构地区：[1]中山大学附属第一医院儿科,广州510080 [2]广州市儿童医院内科 [3]广州中山大学北校区病理教研室

出　　处：《中华儿科杂志》2004年第10期782-786,共5页Chinese Journal of Pediatrics

基　　金：广东省重点科技基金 ( 99M 4815G)

摘　　要：目的　探讨生后早期给予不同饮食构成的营养干预对宫内生长迟缓 (IUGR)幼鼠生长追赶的影响。方法　采用孕母饥饿法建立IUGR大鼠模型 ,32只IUGR新生雌鼠和 8只正常新生雌鼠随机分为五组 (每组 8只 ) :①正常对照组 (C组 ) ,②IUGR模型组 (S组 ) ,③IUGR低蛋白组 (SL组 ) ,④IUGR高蛋白组 (SH组 ) ,⑤IUGR高热卡组 (SA组 )。于生后第 4周测定各组幼鼠的血清胰岛素样生长因子 1(IGF1)及其结合蛋白 3(IGFBP3)浓度和体重、身高、小肠重量、长度及小肠绒毛高度 (VH)、隐窝深度 (CD)、吸收表面积 (VSA)、粘膜厚度 (MT)。结果　IUGR高蛋白组血清IGF1、IGFBP3和小肠VH、VSA、MT及体重均高于C组和S组 (P <0 0 5或P <0 0 1) ;小肠重量、长度和身高与C组比较差异无显著意义 (P >0 0 5 )。血清IGF1与IGFBP3、小肠重量、长度和VH、VSA及体重、身高均呈正相关 (均P <0 0 5 )。结论　血清IGF1是反映生长追赶和营养状态的灵敏指标 ,与小肠和体格的生长追赶呈正相关。生后早期予高蛋白饮食组干预效果较佳 ,可通过促进小肠绒毛的发育和增加营养物质吸收表面积而达到满意的生长追赶。Objective About 20-50% indiv iduals with intrauterine growth retardation (IUGR) could not achieve catch-up g rowth and remain small in size till adulthood. There are few reports on the rel ation between intestinal development and body catch-up growth of IUGR. Studies showed that early 'nutritional programming' would results in long-term eff ects on the body growth and organic function, and gastrointestinal development i s closely related to the body development as well. The authors aimed to study t he effect of early nutritional interventions on serum IGF1, IGFBP3, intestinal d evelopment and catch-up growth of pups with IUGR by using diets with different protein and caloric levels during the first four weeks of life. Meth ods An IUGR rat model was established by maternal nutrition restri ction during pregnancy. Thirty-two IUGR female pups were divided randomly into 4 groups(8 pups in each group)and eight normal female pups as control. The groups and interventions were (1) Normal control group(C group); (2) IUGR contro l group(S group), (3) IUGR low-protein diet group(SL group); (4) IUGR high-pro tein diet group (SH group); (5) IUGR high-caloric group( SA group). The serum IGF1, IGFBP3, body weight, body length, and intestinal weight, length, intestina l villi height(VH), crypt depth(CD), villi absorbing area(VSA), mucous thickness (MT) were measured atthe 4 th week of life. Results (1) At the 4 th week, the serum IGF1 (724.0±153.5 ng/ml), IGFBP3 (9.6 9±3.13 ng/ml),and VH (416.9±46.3 μm), VSA (115.9±24.0×103μm2), MT (583.9±68.5 μm) in the SH group were significantly higher than those of no rmal control group (539.4±198.4 ng/ml, 4.77±2.98 ng/ml and 322.1±25.8 μm, 85.8±17.8×103μm2, 480.0±61.5 μm) and IUGR control group(P< 0.05). The intestinal weight (1.91±0.16 g) and length (80.67±9.47 cm) in the SH group was not significantly different from the normal control group (2. 24±0.22 g and 74.77±9.06 cm, P>0.05). The SH group showed the fastest catch-up growth. Their body weights

关 键 词：IUGR 小肠 幼鼠 IGFBP3 宫内生长迟缓 早期 身高 血清IG 高蛋白 隐窝深度 

分 类 号：R714.5[医药卫生—妇产科学]