血管紧张素转换酶基因多态性与新疆维吾尔族自然长寿的相关性研究  

Polymorphism of angiotensin coverting enzyme gene and natural longevity in the Xinjiang Uygur people:an association study

作　　者：玛依拉.吾甫尔 方鸣武[2] 程祖亨[1] 邱长春[2] 

机构地区：[1]新疆医科大学第一附属医院心内科,乌鲁木齐830054 [2]中国医学科学院基础医学研究所医学分子生物学国家重点实验室

出　　处：《中华医学杂志》2004年第19期1603-1606,共4页National Medical Journal of China

基　　金：国家自然科学基金资助项目 (3 0 160 0 89);美国中华医学基金资助项目 (CMB96 65 7)

摘　　要：目的　探讨血管紧张素转换酶 (ACE)基因插入 /缺失 (I/D)多态性与新疆维吾尔族自然长寿的关系。方法　分别用聚合酶链反应 (PCR)、单链构象多态性分析 (SSCP)和直接测序技术 ,检测百岁组 (≥ 10 0岁 ) 4 2名、90岁组 ( 90 99岁 ) 10 2名、老年组 ( 6 5 70岁 ) 70名老人的ACE基因I/D多态性。以 5 3例 6 5 70岁自然死亡人群为对照。结果　百岁组ACE基因I/D多态基因型D/D、D/I、I/I频率分别为 2 8 6 %、30 9%和 4 0 5 % ,其D和I等位基因频率分别为 4 4 %和 5 6 %。百岁组D/D基因型频率和D型等位基因频率明显高于老年组、对照组 (P <0 0 5、0 0 1) ,与 90岁组比较差异无显著意义 (P>0 0 5 )。结论　初步表明 ,在新疆和田地区维吾尔族 ,ACE基因I/D多态性与个体寿命密切相关 。Objective To explore the distribution of I/D polymorphisms of angiotensin coverting enzyme (ACE) gene and its relation to natural longevity in the Xinjiang Uygur people. Methods Polymerize chain reaction, single strand conformation polymorphism, and direct sequencing technique were used to test the I/D polymorphisms of ACE gene in 42 centenarians, 102 people aged 90 99, 70 people aged 65 70, 53 cases of natural death aged 65 70 were used as controls. Results The frequencies of ACE genotypes DD, DI, and II were 28 6%, 30.9%, and 40 5% respectively in the centenarian group, the frequency rates of D and I alleles were 0 44 and 0 56. The frequency rate of D/D genotype of the centenarian group was significantly higher than that of the group aged 65 70 (28.6% vs. 12.9%,χ 2=4 25, P <0.05), however, not significantly different from that of the group aged 90 ( P >0.05). The frequency rate of D allele of the group of centenarian was significantly higher than that of the group aged 65 70 (44.0% vs. 36.4%, χ 2=4.47, P <0.05). The frequency rates of genotype D/D and D alleles were significantly higher in the centenarian group than in the controls (both P <0.01). Conclusion ACE gene polymorphism is closely correlated with life span of individuals. Longevity is the result of pleiotropic age dependent influence of multiple factors.

关 键 词：长寿 ACE基因 I/D多态性 血管紧张素转换酶基因多态性 老年 相关性研究 新疆维吾尔族 应考 结论 自然 

分 类 号：R181.3[医药卫生—流行病学]