蛋白激酶C激活在高温诱导海马神经细胞凋亡中保护作用的研究  

The protective effect of protein kinase C on apoptosis of hippocampal neurons induced by hyperthermia in rats

作　　者：罗炳德[1] 张培[1] 陈光忠[1] 陈雪梅[1] 邹飞[1] 万为人[1] 

机构地区：[1]第一军医大学高温医学研究室,广东广州510515

出　　处：《中国工业医学杂志》2004年第5期280-282,共3页Chinese Journal of Industrial Medicine

基　　金：军队"十五"指令性课题 (0 1L0 51 )

摘　　要：目的　探讨蛋白激酶C (PKC)在高温诱导的海马神经细胞凋亡中的作用 ,为防治高温致脑损伤提供实验依据。方法　通过建立体外高温诱导海马神经细胞原代培养的凋亡模型 ,应用PKC特异性抑制剂chelerythrinechloride(CTC) ,观察其对细胞凋亡率及对HSP70表达的影响。结果　高温处理后 ,海马神经细胞凋亡率显著升高 ,3 7℃培养加CTC组与正常培养组相比 ,凋亡率差异具有显著性 ;免疫组织化学结果显示 ,高温作用后海马神经细胞内HSP70表达明显升高 ,而应用CTC后 ,其表达则明显下降。结论　高温和CTC均能够诱导海马神经细胞凋亡 ,PKC激活在高温诱导的海马神经细胞凋亡中具有重要的保护作用。Objective To investigate the effects of protein kinase C(PKC)on the apoptosis of hippocampal neurons induced by hyperthermia in neonate rat,thereby to collect information on effective prevention of brain injury.Method To observe the effects of chelerythrine chloride(CTC),a special inhibitor of PKC,on the apoptosis rate and the expression of HSP70,using the hippocampal neurons primary culture apoptosis model induced by hyperthermia in neonate rats. Result After hyperthermia treatment, the apoptosis rate markedly increased,the apoptosis rate of 37 ℃ culture plus CTC treated group was significantly higher than that of 37 ℃ culture group.The immunohistochemistry examination showed that the HSP70 expression was also elevated after hyperthermia, while after application of CTC,the expression decreased obviously.Conclusion Hyperthermia and CTC may all induce the apoptosis of hippocampal neurons in vitro, and PKC activation may play an important protective role in the apoptosis of neurons.

关 键 词：蛋白激酶C 高温 细胞凋亡 海马 HSP70 新生鼠 

分 类 号：R135.3[医药卫生—劳动卫生] R392[医药卫生—公共卫生与预防医学]