用MCF-7细胞检测有机磷农药拟雌激素样活性  

Detection of estrogenic effects of organophosphorus pesticides on MCF-7 human breast cancer cells

作　　者：郑光[1] 周志俊[1] 戴旭峰[1] 孙运光[1] 顾锡安[1] 

机构地区：[1]复旦大学公共卫生学院,上海200032

出　　处：《中国工业医学杂志》2004年第5期273-276,共4页Chinese Journal of Industrial Medicine

基　　金：国家973项目;上海市曙光学者计划基金资助

摘　　要：目的　观察常用有机磷农药对MCF 7细胞增殖以及对雌激素受体基因和pS2基因表达的影响。方法　选用乐果、乙酰甲胺磷、久效磷、马拉硫磷、对硫磷、对氧磷 6种有机磷农药进行MCF 7人乳腺癌细胞增殖实验和转录活化实验。结果　 6种有机磷农药不能诱导MCF 7人乳腺癌细胞增殖 ,但乐果却能使pS2基因表达上调。结论　乐果可能具有拟雌激素样活性 ,且引起pS2基因表达上调不通过雌激素受体介导。Objective To study the estrogenic effects of commonly-used organophosphorus pesticides on the proliferation of MCF-7 cells and the expression of the estrogen receptor(ER)gene and pS2 gene.Method Six kinds of organophosphorus pesticides,dimethoate(Dim),acephate(Ace),monocrotophos(Mon),malathion(Mal),parathion and paraoxon were selected to perform MCF-7 human breast cancer cell proliferation assay and transcription activation assay.Result All these six kinds of organophosphorus pesticides were unable to induce proliferation of human breast cancer MCF-7 cells.And,they could not down-regulate the expression of the ER gene,but only dimethoate could up-regulate the expression of the pS2 gene.Conclusion Dimethoate possibly had estrogenic activity,which could up-regulate the expression of the pS2 gene without mediation of ER.

关 键 词：雌激素样活性 MCF-7人乳腺癌细胞增殖实验 转录活化实验 有机磷农药 

分 类 号：R99[医药卫生—毒理学] Q344.13[医药卫生—药学]