持续电刺激家兔包钦格复合体膈神经放电的观察  

SUSTAINED ELECTRICAL STIMULATION OF BTZINGER COMPLEX ON PHRENIC NERVE DISCHARGES IN RABBITS

作　　者：王桂敏[1] 李勤[1] 于萍[1] 于书彦[1] 张发艳[1] 李艳春[1] 曹莹[1] 宋刚[1] 张衡[1] 

机构地区：[1]山东大学医学院生理研究所,山东济南250012

出　　处：《中国应用生理学杂志》2004年第4期385-388,共4页Chinese Journal of Applied Physiology

基　　金：教育部<高等学校骨干教师资助计划>项目;山东省卫生厅青年基金资助项目(200321);山东大学医学院科研基金资助项目(2003)

摘　　要：目的观察长时持续电刺激家兔Btzinger复合体(Bt.C)对膈神经放电的影响。方法采用10～50μA,40～100Hz,波宽0.3ms的刺激参数,对30只家兔Bt.C进行15～30s的刺激。结果刺激期间,出现膈神经放电的抑制或“切断”,即吸气相减弱或消失;随刺激强度和频率的增加,膈神经放电被抑制的程度逐渐增强;刺激Bt.C所致的吸气抑制存在“习惯化”现象,表现为一定刺激范围内,随刺激时间的延长,膈神经放电积分幅度呈“阶梯”状逐步上升;停止刺激后出现膈神经放电的“反弹”,主要表现为放电幅度明显升高,而后逐渐恢复正常;膈神经放电的“习惯化”具有一定的短时记忆效应。结论非联合性学习和记忆形式存在于Bt.C对呼吸的调节中,与Bt.C有关的神经元突触在功能上具有一定的可塑性。Aim:To observe the effects of sustained electrical stimulation at Btzinger complex(Bt.c) on phrenic nerve discharges. Me thods: Sustained electrical stimulation (10～50 μA,40～100 Hz,0.3 ms,for 15～30 s)of Bt.C on 30 urethane anaesthetized, vagotomized, paralyzed and artificially ventilated rabbits. Results: Sustained electrical stimulation of Bt.C produced the inhibition or “inspiratory off switch” of phrenic discharge during the stimulation. The inhibition of the phrenic discharges showed intensity and frequency dependence. Habituation was shown during the stimulation, showing the magnitude of the phrenic nerve discharge increased gradually. Post stimulus rebound exhibited upon the cessation of the stimulation, showing the magnitude of the phrenic activity increased significantly. Short term memory was shown in the habituation of the phrenic activity. Conclusion: Non associative learning is involved in the central control of respiratory modulation in the Bt.C and synaptic plasticity may exist in the respiratory neurons of Bt.C.

关 键 词：Botzinger复合体 膈神经放电 电刺激 习惯化 家兔 

分 类 号：Q424[生物学—神经生物学]