L-选择蛋白可能介导小鼠肝癌HCa-F细胞经淋巴道转移的研究  

THE EXPRESSION OF L-SELECTIN FACILITATES MURINE HEPATOMA CELL LINE HCa-F'S METASTASIS THROUGH LYMPH NODE

作　　者：刘敏[1] 王莉芬[2] 朱杰[3] 杨佩满[4] 张嘉宁[1] 

机构地区：[1]大连医科大学生物化学教研室,大连116027 [2]大连医科大学附属第二临床医院病理科 [3]大连医科大学附属第二临床医院检验科 [4]大连医科大学组织学胚胎学教研室,大连116027

出　　处：《解剖学报》2004年第5期502-508,共7页Acta Anatomica Sinica

基　　金：辽宁省教委资助项目 ( 990 2 2 10 61;2 0 2 2 0 3 2 47) ;教育部优秀青年教师资助计划项目 ;教育部骨干教师资助计划项目

摘　　要：目的　观察L 选择蛋白是否介导小鼠肝癌HCa F细胞与淋巴结黏附 ,探讨HCa F细胞特异性向周围淋巴结转移的分子机制。　方法　应用免疫细胞化学方法验证小鼠肝癌HCa F细胞为非淋巴细胞来源 ;用免疫印迹分析、RT PCR及流式细胞术检测L 选择蛋白在HCa F细胞的表达 ;并用L 选择蛋白的抗体抑制HCa F细胞与冰冻淋巴结切片间的黏附实验 (Stamper Woodruffmethod)检测L 选择蛋白的功能。　结果　HCa F细胞为非淋巴细胞来源的肝癌细胞 ,在其表面有L 选择蛋白的表达 ,且HCa F细胞与淋巴结之间的黏附可被L 选择蛋白的抗体抑制。结论　非淋巴细胞来源的HCa F细胞表面表达功能性L 选择蛋白分子 ,并可协助其与淋巴结黏附 ,从而可能介导HCa F细胞向淋巴道转移。Objective L-selectin is an important adhesion molecule,which is the lymph cell's homing receptor specifically expressed on leukocytes.A non-lymphoma cell line-HCa-F has the high potential ability of metastasis to peripheral lymph nodes,whose character is similar to lymphoma's.We want to explore whether the HCa-F cell's metastasis mechanism is also similar to lymphoma's,that is to say,whether HCa-F cell can also express L-selectin and the expression of L-selectin bring about the lymph node metastasis character. Methods First,α-fetoprotein,CD3 and CD20's monoclonal antibodies were used to identify the origin of HCa-F cell;then we analysed whether the HCa-F cell can express L-selectin by means of flow cytometry analysis,RT-PCR,and Western blotting;finally,Stamper-Woodruff method was used to examine the L-selectin's function. Results Immunostaining showed that the HCa-F cell was α-fetoprotein positive,CD3 and CD20 negative cell line,which suggests that it is a liver cell line.And results of flow cytometry,RT-PCR,and Western blotting showd that HCa-F cell can express L-selectin.Additionally,anti-L-selectin's antibody can inhibit the adhesion between HCa-F cells and frozen section of mouse lymph node.Conclusion L-selectin expressed on HCa-F cells is a functional molecule which can facilitate HCa-F cell's metastasis to lymph nodes.

关 键 词：L-选择蛋白 肿瘤转移 HCa-F细胞 615小鼠 

分 类 号：Q513.2[生物学—生物化学]