信号转导子与转录激活子3和细胞因子信号转导抑制因子-3蛋白在成年大鼠脊髓内的表达与定位  

EXPRESSION AND LOCALIZATION OF STAT3 AND SOCS-3 IN ADULT RAT SPINAL CORD

作　　者：赵靖[1] 石献忠[1] 刘猛[1] 

机构地区：[1]北京大学医学部解剖学系,北京100083

出　　处：《解剖学报》2004年第5期537-540,共4页Acta Anatomica Sinica

基　　金：北京大学 2 0 0 1 985青年科研启动基金资助项目

摘　　要：目的　分析JAK STAT信号转导途径重要成员STAT3蛋白和该途径抑制蛋白SOCS 3在大鼠脊髓内的表达和细胞内定位分布。　方法　免疫细胞化学技术和形态计量学方法。　结果　STAT3免疫阳性产物主要分布于脊髓前角运动神经元的胞浆内 ;SOCS 3免疫阳性产物广泛分布于脊髓前角及后角神经元内、部分胶质细胞及神经纤维内。在前角运动神经元内 ,SOCS 3免疫阳性产物主要分布于核内 ,有时也可分布于胞浆中。　结论　STAT3和SOCS 3广泛表达于正常大鼠脊髓内 ,其中SOCS 3具有核蛋白或胞浆蛋白两种存在形式。Objective To study the expression and cellular localization of STAT3 and SOCS-3 in the motor neurons of normal rat spinal cord. Methods Immunocytochemistry and morphometry methods were used in the present study. Results STAT3 immunocytochemical staining was mainly distributed in the cytoplasm of the motor neurons of the ventral horns, SOCS-3 immunoreactive products were extensively distributed in the neurons of the ventral and dorsal horns, glia and fibers of the spinal cord. In the ventral horn, SOCS-3 immunoreactivity was found in the nuclei and/or cytoplasm of the motor neurons.Conclusions STAT3 and SOCS-3 are extensively distributed in the normal adult rat spinal cord and SOCS-3 is existence in the forms of nuclear or cytoplasmic protein.

关 键 词：信号转导子与转录激活子-3 细胞因子信号转导抑制因子-3 Janus激酶-信号转导子与转录激活子 脊髓 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]