人类新基因cegl cDNA的克隆及表达研究  

CLONING AND CHARACTERIZATION OF A NOVEL HUMAN ceg1 GENE cDNA

作　　者：付佳琪[1,2,3] 王峦[1,2,3] 陈微 慈宏亮[1,2,3] 李亦平[1,2,3] 

机构地区：[1]北京师范大学生命科学学院生物医学研究所,北京100875 [2]浙江赛尔生物医学研究院,杭州310006 [3]美国哈佛大学牙医学院Forsyth研究所,波士顿02115

出　　处：《实验生物学报》2004年第5期409-417,共9页Acta Biologiae Experimentalis Sinica

摘　　要：CLECT and EGF-like domain contained Gene l(cegl)基因是用电子克隆的方法获得的人类新基因。该基因定位在人类的第14号染色体上,是一个单一外显子的基因。cegl基因的cDNA长度为2050bp,通过生物信息学方法预测它包含一个1340bp的完整阅读框架,编码一个490个氨基酸的蛋白,含有CLECT、EGF-like结构域各一个。以cegl基因全长编码区为探针的整体原位杂交结果显示该基因的小鼠和鸡的同源基因在各自早期胚胎头部中特异表达,并且在不同时期胚胎神经系统增殖迅速的部位中有大量的表达。RT-PCR结果显示该同源基因在小鼠成体各组织中广泛分布。这提示cegl基因可能与头部生长发育有密切关系,并且对维持成体各组织的正常功能起到重要的作用。对cegl基因在胚胎发育的时间和空间表达模式的研究将有助于进一步深入地揭示它在人脑的正常生长发育中的作用。cegl gene was a novel human gene,which was cloned by bioinformatics research and RT- PCR.It was a single exon gene and located on human chromosome 14.The length of the cDNA was 2050bp.Bioinformatics analysis predicted a 1340 bp complete open reading fi-ame(ORF)which enceded a 446 amino acid protein,containing an EGF-like and CLECT domain.We found that the homologous genes of cegl in mouse embryo and chicken embryo were specifically expressed in the brain by in-situ hybridization. The resuit of RT-PCR of the mature mouse organs showed it was widely expressed in many organs.The re- suh indicates that cegl gene may have an essential role in the development of brain and the maintenance of the organs'normal function.The analysis of expression and function profile of cegl gene may provide valuable insights into the functions of cegl in the development and fimction of human body.

关 键 词：正常 EGF 表达 小鼠 新基因 头部 人类 l基因 成体 生长发育 

分 类 号：Q78[生物学—分子生物学] S643.1[农业科学—蔬菜学]