在体监测吲哚氰绿在肿瘤中引起的X射线衰减  

In Vivo Observing X-Ray Attenuation of Intratumor Injection of Indocyanine Green

作　　者：叶昶[1] 骆清铭[1] 梁文锡[1] 陆锦玲[1] 

机构地区：[1]华中科技大学生物医学光子学教育部重点实验室,武汉430074

出　　处：《光学学报》2004年第10期1437-1440,共4页Acta Optica Sinica

基　　金：国家自然科学基金(60178028);国家杰出青年科学基金(60025514);教育部科学技术研究重大项目(重大10420);211工程资助课题

摘　　要：介绍了在体观测肿瘤内注射吲哚氰绿 (ICG) ,一种激光吸收染剂前后X射线衰减的实验结果。实验采用已种植两周的骨髓瘤细胞 (SP2 / 0 ) ,质量为 15～ 2 0g的BALB/c小鼠作为动物模型 ,采用数字X射线成像系统作为监测药物在肿瘤内扩散的机制。系统工作在 33kVp ,0 .3mA下 ,曝光时间 4s,采用 1.5×放大倍率。实验研究的是通过灰度值反应出不同区域的X射线衰减 ,和周围正常组织和图像中没有物体的背景区域相比较 ,注射ICG前后肿瘤内的灰度值发生了明显的变化。实验证明 ,使用数字X射线成像系统能够动态、有效、非侵入地监测ICG在肿瘤组织内的扩散 ,从而达到优化治疗结果的作用。Some experimental results of in vivo observing X-ray attenuation of intra-tumor injection of indocyanine green (ICG) are reported. An eight-to nine-week-old male BALB/c mouse with mass between 15 g and 20 g is used in the experiments. The mouse is implanted with myeloma cell line (SP2/0) two week before. A system used to monitor the intratumor diffusion of ICG is a digital X-ray imaging system. It works at 33 kVp, 0.3 mA with exposures duration of 4 seconds and magnification of 1.5. The objective of this research is to study the X-ray attenuation at different area, which represented by gray-scale value. Compare to the ROI in the tissue without ICG and ROI of black background in the image, there is an obvious change in the tumor, which is the area ICG can diffuse to. It shows the feasibility of using digital X-ray imaging system dynamically, effectively and noninterventionly to monitor the diffusion of the ICG.

关 键 词：X射线光学 在体监测 X射线衰减 数字X射线成像 吲哚氰绿 

分 类 号：Q81[生物学—生物工程] R8[医药卫生—临床医学]