牛膝甾酮25位差向异构体的分离与鉴定  

Isolation and structure identification of C-25 epimers of inokosterone from Achyranthes bidentata Blume.

作　　者：朱婷婷[1] 梁鸿[1] 赵玉英[1] 王邠[1] 

机构地区：[1]北京大学药学院天然药物学系,北京100083

出　　处：《药学学报》2004年第11期913-916,共4页Acta Pharmaceutica Sinica

摘　　要：目的　分离并确定苋科植物怀牛膝 (AchyranthesbidentataBlume .)中牛膝甾酮 2 5位差向异构体的结构。方法　利用色谱技术分离纯化牛膝甾酮 2 5位差向异构体 ,用波谱 (IR ,UV ,MS ,NMR)方法及化学方法确定结构。结果　从怀牛膝的乙酸乙酯部位分离得到 3个化合物 ,分别鉴定为 2 5S 牛膝甾酮 (1,2 5S inokosterone) ,2 5R 牛膝甾酮 (2 ,2 5R inokosterone) ,β 蜕皮甾酮 (3,ecdysterone)。 结论　化合物 1和 2为首次从怀牛膝中分离得到的 2 5位差向异构体 ,首次确定了 2 5位绝对构型和发表该 2 5位差向异构体的1 3CNMR数据。Aim To isolate C-25 epimers of inokosterone from Achyranthes bidentata Blume. and identify their structures. Methods To separate C-25 epimers of inokosterone by using various kinds of chromatography methods and identify their structures on basis of spectral analysis and chemical method. Results Three compounds were isolated and their structures were established as 25S-inokosterone (1), 25R-inokosterone (2) and ecdysterone (3). Conclusion Compounds 1 and 2 are new C-25 configuration isomers from Achyranthes bidentata Blume., their absolute configurations are elucidated at the first time, and their 13CNMR data are reported for the first time.

关 键 词：怀牛膝 25R-牛膝甾酮 25S-牛膝甾酮 β-蜕皮甾酮 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]