FHIT基因在上皮性卵巢肿瘤中的表达及其临床意义  

The expression of fragile histidine triad gene in epithelial ovarian tumors and its clinical significance

作　　者：胡叶青[1] 刘元姣[1] 

机构地区：[1]武汉大学人民医院妇产科,430060

出　　处：《实用医学杂志》2004年第11期1234-1236,共3页The Journal of Practical Medicine

摘　　要：目的 :检测脆性组氨酸三联体蛋白 (FHIT蛋白 )在上皮性卵巢肿瘤中的表达 ,探讨其表达与上皮性卵巢癌临床病理参数之间的关系 ,以期了解其表达在鉴别卵巢良、恶性肿瘤 ,判断恶性肿瘤预后中的意义。方法 :收集我院 94例上皮性卵巢肿瘤的外科手术石蜡标本 (2 0例良性、18例交界性、5 6例恶性 ) ,用免疫组化SP的方法检测FHIT蛋白的表达。并分析FHIT蛋白的表达与年龄、病理分级、分类、临床分期以及有无淋巴结转移的关系。结果 :FHIT蛋白在 2 0例良性上皮性肿瘤及 18例交界性肿瘤中均无阴性表达 ,而在 5 6例恶性上皮性肿瘤中有 13例FHIT蛋白阴性表达 (2 3 2 1% )。而且FHIT蛋白阴性表达癌在癌组织学分级中的分布为Ⅰ级 0 /16例 ,Ⅱ～Ⅲ级 13 /4 3例 ,两组比较差异有显著性 (P <0 0 5 ) ;FHIT蛋白阴性表达率在伴淋巴结转移组和不伴淋巴结转移组间比较差异也有显著性 (P <0 0 5 ) ;而FHIT蛋白的阴性表达率与年龄、病理类型及临床分期无关。结论 :FHIT蛋白只在上皮性卵巢癌中特异性表达丧失 ,而且在上皮性卵巢癌中其表达丧失可能与高病理分级、淋巴结转移阳性相关 ,提示FHIT蛋白表达丧失在上皮性卵巢癌的发生和发展中起重要作用 ,其表达对卵巢上皮性肿瘤良恶性鉴别及预后判断具有临床指导意义 ,FHIT蛋白表达?Objective To investigate the express io n of fragile histidine triad (FHIT) protein and the possible relationship betwee n FHIT expression and clinicopathological parameters in epithelial ovarian tumor s. Methods Detect FHIT protein expression in 94 cases of formali n-fixed, paraffin-embedded epithelial ovari an tumors by SP immunohistochemistry method, and analyze its relation to a ge, histological grade ,clinical stage, histological type and lymph node metasta sis. Results None of 20 cases of benign epithelial ovarian tumors and 18 cases of borderline epithelial ovarian tumors showed loss of FHIT protein expression. But 13 of 56 c ases(23.21%) of epithelial ovarian carcinomas lost the expression of FHIT protei n. The distribution of negative expression of FHIT protein was 0/16 in gradeⅠ, 13/43 in grade Ⅱ-Ⅲ respectively. There was significant difference(P<0. 05).The negative expression of FHIT protein was 6/42 in negative lymph node met astasis, 7/14 in positive lymph node metastasis. There was significant differenc e(P<0.05), too. However, the negative expression of FHIT protein was not correlated with different age, clinical stage and histological type groups( P>0.05). Conclusion The loss of FHIT protein only specifically occurs in epithelial ovarian carcinom as. The rate of negative FHIT protein expression may be associated with advanced -grade and positive lymph node metastasis. It is suggested that loss of FHIT pr otein expression play an important role in the development and progression of th e epithelial ovarian tumors, which may indicate malignant tumors and poor p rognosis.

关 键 词：FHIT基因 上皮性卵巢肿瘤 基因表达 免疫组织化学 免疫组化 

分 类 号：R737.31[医药卫生—肿瘤]