Kv1.2、Kv1.5、Kv2.1钾通道在缺氧性肺血管收缩中的作用  

Roles of the Kv1.2, Kv1.5, Kv2.1 potassium channels in hypoxia pulmonary vasoconstriction

作　　者：杜以梅[1] 唐明[1] 刘长金[1] 洪志刚[1] 柯琴梅[2] 狄久芳 骆红艳[1] 胡谋先[1] 胡新武[1] 席姣娅[1] 唐碧[1] Jurgen Hescheler 

机构地区：[1]华中科技大学同济医学院生理系 [2]华中科技大学同济医学院附属协和医院老年病科 [3]滕州中医院麻醉科 [4]Institute of Neurophysiology,University of Cologne,D-50931 Cologne,Germany

出　　处：《中国病理生理杂志》2004年第9期1537-1541,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.30200098);湖北省自然科学基金资助项目(No.2004ABA191)

摘　　要：目的和方法雄性Wistar大鼠随机分为两组常氧对照组和低氧组。用酶消化的方法获得单个大鼠肺内动脉平滑肌细胞(PASMC)。采用全细胞膜片钳技术,记录PASMC静息膜电位(Em)和电压门控性钾通道电流(IKv),通过细胞内灌流Kv12/Kv15/Kv21抗体混合液(1∶125),探讨Kv12、Kv15、Kv21钾通道在缺氧性肺血管收缩(HPV)中的作用。结果①低氧组膜电位明显去极化,由(-518±08)mV去极到(-472±07)mV,P<001,IKv与常氧组相比显著降低,在测试电压-30mV时,IKv由(616±058)pA/pF降为(331±037)pA/pF(P<001)。②细胞内灌流Kv12/Kv15/Kv21抗体混合液可显著抑制常氧对照组PASMC的IKv,使Em去极化,然而细胞内灌流Kir21/Kir23/Kir41(1∶125)抗体混合液对常氧对照组PASMC的IKv和Em无显著影响。③细胞内灌流Kv12/Kv15/Kv21抗体混合液和Kir21/Kir23/Kir41抗体混合液对低氧组PASMC的IKv和Em均无显著影响。结论Kv12、Kv15、Kv21可能是氧敏感型通道,并介导了低氧性肺血管收缩。AIM:To determine the role of Kv1 2, Kv1 5, Kv2 1 in the hypoxia pulmonary vasoconstriction (HPV). METHODS: Male Wistar rats were divided into two groups: normoxic group and hypoxic group. The single smooth muscle cell was obtained from pulmonary artery of Wistar rats with acute enzymatic digestion method. The conventional whole-cell patch clamp technique was used to record the resting membrane potential (Em) and the potassium currents of voltage-gated potassium channel (IKv) in rat pulmonary arterial smooth muscle cells (PASMC). Intracellular application of Kv1 2/Kv1 5/Kv2 1 antibodies (1∶125) was conducted through the whole-cell patch clamp system. RESULTS: ① Em of PASMC was depolarized after 24 h hypoxia compared with that of control cells [from (-51 8±0 8) mV to (-47 2± 0 7) mV, P <0 01]. IKv of PASMC was decreased after 24 h hypoxia, [from (6 16±0 58) pA/pF to (3 31±0 37) pA/pF at -30 mV, P <0 01]. ② The mixture of Kv1 2/Kv1 5/Kv2 1 antibodies depolarized Em and inhibited IKv in PASMC from normoxic rat, whereas the mixture of Kir2 1/Kir2 3/Kir4 1 antibodies had no effects on them. ③ The mixture of Kv1 2/Kv1 5/Kv2 1 antibodies and the mixture of Kir2 1/Kir2 3/Kir4 1 antibodies had no effects on IKv and Em from rats hypoxic for 24 h. CONCLUSION: Kv1 2, Kv1 5, Kv2 1 might be oxygen sensitive potassium channels which mediated HPV. [

关 键 词：肺动脉 钾通道 膜片钳术 抗体 

分 类 号：R331.32[医药卫生—人体生理学]