糖尿病大鼠心肌肌浆网钙调蛋白基因表达的改变  

Gene expression of proteins handling the calcium homeostasis in the diabetic rat heart

作　　者：周斌全[1] 胡申江[2] 

机构地区：[1]浙江大学医学院附属邵逸夫医院心内科,浙江杭州310016 [2]浙江大学医学院附属第一医院心内科,浙江杭州310003

出　　处：《中国病理生理杂志》2004年第9期1593-1596,共4页Chinese Journal of Pathophysiology

基　　金：国家教育部留学回国人员科研基金资助(No.1999-747)

摘　　要：目的探讨糖尿病大鼠心肌细胞肌浆网钙调蛋白基因表达的改变。方法雄性SD大鼠经尾静脉注射四氧嘧啶(40mg/kg)复制糖尿病大鼠模型,对照组注射生理盐水,分别于4,6周处死,取心室肌组织,应用逆转录-聚合酶反应技术以肌动蛋白为内参照,测定肌浆网钙调蛋白钙泵(SERCA)、磷酸受纳蛋白(phospholamban)、ryanodine受体2型(RyR2)、1,4,5三磷酸肌醇受体2型(IP3R2)mRNA的变化。结果4,6周后糖尿病组大鼠的体重和心脏重量均低于正常对照组,但心脏/体重比显著大于正常对照组。4周糖尿病组大鼠心肌肌浆网SERCA、磷酸受纳蛋白、RyR2、IP3R2mRNA的表达与对照组相比无显著差异;6周的糖尿病组大鼠心肌肌浆网Ca2+-ATP酶的mRNA表达无明显变化,但磷酸受纳蛋白的mRNA表达显著高于而RyR2、IP3R2的mRNA表达显著低于对照组。结论糖尿病大鼠心肌细胞的肌浆网磷酸受纳蛋白的mRNA表达增加,RyR2、IP3R2的mRNA表达下降。AIM: To investigate the gene expression of proteins handling the calcium homeostasis in diabetic rat heart. METHODS: Diabetes was induced in male Sprague-Dawley rats (180-220)g by administering a single tail-vein injection (40mg/kg ) of alloxan, and the control group was injected with normal saline. At the end of four, six weeks after the induction of diabetes, the animals were killed for assessment of the ventricular gene expression of proteins handling the calcium homeostasis which was measured by reverse transcription-polymerase chain reaction and nomalized to the mRNAs level of actin. RESULTS: At the end of 4 and 6 weeks, the weight of body and the heart in the diabetic group is lower than that in control rats, heart to body weight of diabetic rats is higher than control. No changes in mRNAs level of sarcoplasmic reticulum calcium ATPase, phospholamban, ryanodine receptor type 2, 1,4,5-trisphosphate inositol receptor type 2 were observed 4 weeks after alloxan injection. Expression of phospholamban mRNAs was significantly increased, but ryanodine receptor type 2, 1,4,5-trisphosphate inositol receptor type 2 mRNAs were significantly decreased than that in the age-matched controls. In contrast, expression of mRNAs coding sarcoplasmic reticulum calcium ATPase was not affected 6 weeks after alloxan injection. CONCLUSION: Gene expression of phospholamban is increased while ryanodine receptor type 2, 1,4,5-trisphosphate inositol receptor type 2 are decreased in the diabetic rat heart. [

关 键 词：糖尿病 肌浆网 钙调蛋白 基因表达 

分 类 号：R587.1[医药卫生—内分泌]