丹参、三七的有效部位对血瘀证大鼠血小板粘聚性及TXA_2、PGI_2的影响  

Effects of Active Fractions of Danshen or Sanqi on Platelet Adhesion,Aggregation and TXA_2,PGI_2 in Rat Model of Blood Stasis Syndrome

作　　者：杨佳[1] 秦彩玲[1] 刘婷[1] 张毅[1] 孙有富[1] 谭洪根[1] 张淑运[1] 张伯礼[2] 

机构地区：[1]中国中医研究院中药研究所,北京100700 [2]天津中医药大学,天津300193

出　　处：《中国实验方剂学杂志》2004年第6期35-38,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点基金研究发展规划项目(G1 9990 5440 3)

摘　　要：采用皮下注射肾上腺素加冰水冷浴法造大鼠血瘀模型 ,观察丹参、三七的有效部位丹酚酸B、Rg对血小板粘聚性及TXB2 、6 Keto PGF1α的含量的影响。结果表明 :大鼠肾上腺素血瘀模型组与正常对照组比较 ,血浆血小板聚集性 (ADP诱导 )、粘附性均显著升高 ,血浆中TXB2 、6 Keto PGF1α水平及TXB2 /6 Keto PGF1α比值均无显著影响 ,对ADP诱导血小板生成的TXB2 、腹主动脉组织的 6 Keto PGF1α的含量及TXB2 /6 Keto PGF1α比值亦无显著影响。有效部位丹酚酸B、Rg对肾上腺素血瘀模型大鼠血小板粘聚性升高有明显抑制作用。有效部位丹酚酸B、Rg对肾上腺素血瘀模型大鼠血浆中TXB2 、ADP诱导血小板生成TXB2 及血浆中 6 Keto PGF1α、腹主动脉组织 6 Keto PGF1α均有明显升高的作用 ,对TXB2 6 Keto PGF1α均无明显影响。结论 :此血瘀模型复制成功 ,推测其升高血小板聚集性的机理与TXA2 PGI2 系统无明显关系。有效部位丹酚酸B、Rg对肾上腺素血瘀模型大鼠血小板粘聚性抑制作用机理推测主要与升高 6 KetoThe rat model of blood stasis syndrome was caused by subcutaneous adrenaline injection incorporated with ice-bathing.We studied the effects of the active fractions of Danshen and Sanqi,i.e.salvianolic acid B(Sal.B)and Rg,on platelet aggregation,platelet adhesion,TXA 2 and PGI 2 in rat model of blood stasis syndromes.The results indicated that compared with normal rats,the platelet adhesion and ADP-induced platelet aggregation were markedly increased.However, the plasma levels of TXB,6-keto-PGF 1α and ADP-induced platelet products of TXB 2 and 6-keto-PGF 1α in abdominal aorta were not markedly affected in the model rats.Sal.B and Rg significantly inhibited the increase in platelet adhesion and ADP-induced platelet aggregation in the model rats.Sal.B and Rg also markedly increased the plasma levels of TXB 2, 6-keto-PGF 1α and ADP-induced platelet production of TXB 2 and 6-keto-PGF 1α in abdominal aorta of the model,but had little effect on the ratio of TXB 2/6-keto-PGF 1α .The results indicated that the blood stasis syndrome model was established successfully.We inferred that the changes in platelet aggregation and platelet adhesion might not be related with TXA 2-PGI 2.Moreover,the other conclusion is that the inhibition effects of Sal.B and Rg of Danshen and Sanqi in Fufangdanshen prescription on platelet aggregation and adhesion might be closely related to its promotive effect on the release of 6-Keto-PGF1α in the rats.

关 键 词：丹酚酸B Rg 血小板聚集性 TXB2 6-KETO-PGF1Α 

分 类 号：R285.5[医药卫生—中药学]