原钒酸钠对2型糖尿病大鼠骨骼肌GLUT4mRNA表达的影响  

Effect of sodium orthovanadate on GLUT4 mRNA expression in the skeletal muscle of type 2 diabetic rats

作　　者：王宁[1] 艾静[1] 杨梅[1] 方志伟[1] 杜智敏[2] 杨宝峰[1] 

机构地区：[1]哈尔滨医科大学药理学教研室,黑龙江哈尔滨150086 [2]哈尔滨医科大学第二临床医学院临床药理教研室,黑龙江哈尔滨150086

出　　处：《中国药学杂志》2004年第11期828-830,共3页Chinese Pharmaceutical Journal

基　　金：黑龙江省科技厅重大项目基金资助课题(No.20010101001-00)

摘　　要：目的 观察原钒酸钠(sodium orthovanadate)对2型糖尿病大鼠骨骼肌葡萄糖转运蛋白4(GLUT4)mRNA表达的影响。方法 用高脂饲料灌胃正常大鼠10 d,引起肥胖,再用四氧嘧啶(120mg·kg-1)腹腔注射,建立2型糖尿病大鼠模型。筛选空腹血糖值大于16.7 mmol·L-1的大鼠,随机分成5组:原钒酸钠大剂量组(9 mg·kg-1·d-1)、原钒酸钠中剂量组(3 mg·kg-1·d-1)、原钒酸钠小剂量组(1 mg·kg-1·d-1)、糖尿病模型组、苯乙双胍组(75 mg·kg-1·d-1)。连续用药7 d,用快速血糖仪测空腹血糖值。用胰岛素放免试剂盒测血清胰岛素值。用骨骼肌GLUT4原位杂交试剂盒检测骨骼肌GLUT4 mRNA的表达。结果①2型糖尿病大鼠经原钒酸钠灌胃给药后,空腹血糖值与模型组相比有明显的降低(P<0.05);②用药组大鼠血清胰岛素值与模型组相比无显著差异;③用药组大鼠的骨骼肌GLUT4 mRNA表达量与模型组相比均有明显增多。结论 原钒酸钠对2型糖尿病大鼠有明显的降糖作用,对血清胰岛素的分泌无明显影响,对2型糖尿病大鼠骨骼肌GLUT4 mRNA的表达有促进作用。OBJECTIVE: To study the effect of sodium orthovanadate on glucose transporter 4(GLUT4) mRNA expression in the skeletal muscle of type 2 diabetic rats. METHODS: The rats were feed with high-fat food for 10 days to get obese. Alloxan (120 mg&middotkg-1) was given to the rats by ip, establishing type 2 diabetes model. The rats with plasma glucose levels higher than 16.7 mmol&middotL-1 were selected, and organized into 5 groups randomly: sodium orthovanadate high dose group (9 mg&middotkg -1&middotd-1), middle dose group (3 mg&middotkg -1&middotd-1), low dose group (1 mg&middotkg -1&middotd-1), diabetes model group, and meltrol group (75 mg&middotkg-1&middotd-1). The rats in sodium orthovanadate groups were administered for 7 days, and then the levels of plasma glucose and insulin were assayed. GLUT4 mRNA expression in the skeletal muscle was determined by the hybridization in situ technique. RESULTS: After fed with sodium orthovanadate, the plasma glucose levels of the type 2 diabetic rats were obviously lower than those of the diabetes model rats(P < 0.05). There was no obvious difference for the plasma insulin value between the sodium orthovanadate group and the diabetes model rats. GLUT4 mRNA expression in the skeletal muscle of the diabetic rats fed with sodium orthovanadate was obviously greater than that of the diabetes model rats. CONCLUSION: The study suggested that sodium orthovanadate can decrease plasma glucose level, and promote GLUT4 mRNA expression in the skeletal muscle of the type 2 diabetic rats.

关 键 词：原钒酸钠 胰岛素 骨骼肌 葡萄糖转运蛋白4 

分 类 号：R965[医药卫生—药理学]