甲磺酸罗哌卡因对映体的毛细管电泳法拆分研究  

Study on the enantioseparation of ropivacaine mesylate by capillary zone electrophoresis using 2,3,6-trimethyl-β-cyclodextrins as chiral additives

作　　者：刘言[1] 黄志东[1] 

机构地区：[1]天津市药品检验所,天津300070

出　　处：《中国药学杂志》2004年第11期856-858,共3页Chinese Pharmaceutical Journal

摘　　要：目的 以三甲基-β-环糊精为手性选择剂,研究甲磺酸罗哌卡因对映体拆分方法。方法 用区带毛细管电泳模式。探讨三甲基-β-环糊精对甲磺酸罗哌卡因对映体的拆分的影响,确定拆分甲磺酸罗哌卡因对映体的最佳条件,并与β-环糊精、羟丙基-β-环糊精进行了对比。结果 以β-环糊精和羟丙基-β-环糊精作为手性选择剂时,不能使甲磺酸罗哌卡因对映体达到基线分离,而三甲基-β-环糊精能获得很好的分离结果。结论 本方法简便、快速,能比较准确地测定甲磺酸罗哌卡因对映体的纯度。OBJECTIVE: To investigate the enantioseparation of ropivacaine mesylate using capillary electrophorsis (CE) with three cyclodextrins as the chiral additives. METHODS: The enantioseparation of ropivacaine mesylate was reported with β-CD, HP-β-CD and TM-β-CD as chiral selectors employing an uncoated capillary. The effects of the pH, composition and concentration of the running buffer solution, the kind and concentration of chiral selectors, the running voltage, the capillary temperature on the mobility and chiral separation were studied. RESULTS: TM-β-CD showed a baseline enantiomeric separation for ropivacaine, whereas the β-CD and HP-β-CD, under optimal conditions, was poor in stereoselectivity and showed just partial chiral separation. CONCLUSION; The enantioseparation is an accurate, reliable and convenient method for measuring enantiomeric purity of ropivacaine mesylate.

关 键 词：毛细管区带电泳 对映体分离 甲磺酸罗哌卡因 

分 类 号：R927.1[医药卫生—药学]