大鼠出生后不同发育阶段大脑海马CA_3区PCNA和NGF表达的研究  

STUDY ON THE EXPRESSION OF PCNA AND NGF IN CA_3 AREA OF HIPPOCAMPUS ATDIFFERENT POSTNATAL GROWTH STAGES OF RATS

作　　者：杜金凯[1] 庞晓静[1] 高福禄[1] 

机构地区：[1]承德医学院,河北承德067000

出　　处：《承德医学院学报》2004年第4期286-288,共3页Journal of Chengde Medical University

摘　　要：目的 :通过对大鼠出生后海马CA3 区PCNA和NGF表达的研究 ,以期寻找出大脑海马的发育规律。方法 :取生后第 1天、第 2天、第 3天、第 5天、第 10天、第 2 0天、第 30天大鼠脑 ,10 %福尔马林固定 ,免疫组化染色 ,观察PCNA、NGF在大脑海马CA3 区表达的变化情况。结果 :大鼠出生后海马CA3区细胞增殖以第 5天最为明显 ,其中多形层细胞增殖数量最多 ,其次是分子层 ,锥体层细胞增殖最少 ,第10天以后逐渐减少 ,第 30天时基本不再有增殖。NGF在第 1～ 5天时未见表达 ,第 10天开始出现表达 ,其中以锥体层阳性表达最多 ,第 2 0天时基本稳定。结论 :NGF的表达对神经细胞的生存有支持作用。Objective:By studying on the expression of PCNA and NGF in CA 3 area of hippocampus to make out the developmental law of hippocampus . Method: The brain of rats were taken out at postnatal d1,d2,d3,d5,d10,d20,d30 respectively and fixed with 10% formalin, SP immunohistochemistry was used in this study to observe the expression of PCNA,NGF in CA 3 area of hippocampus .Results: The PCNA expression in CA 3 area of hippocampus was the most obvious at postnatal d5:neurons in multiform layer proliferated the most, multiform layer took second place and pyramidal layer the least . The PCNA expression decreased gradually after postnatal d10 and basically ended at postnatal d30. NGF positive cell was not observed in CA 3 area of hippocampus at postnatal d1～d5.The expression of NGF reached the top at postnatal d10 and expressed the most in pyramidal layer and became stable at postnatal d20. Conclusions: The expression of NGF has supporting functions to neurons.

关 键 词：海马 发育 神经生长因子 免疫组化 大鼠 

分 类 号：R329.1[医药卫生—人体解剖和组织胚胎学]