OTS-2.2S基因芯片对人脑挫伤后基因表达差异的初步研究  

A primary study on the gene expression profiling of human brain contusion by cDNA microarray

作　　者：周亦武[1] 张益鹄[1] 刘艳[1] 邓伟年[1] 王成毅[1] 

机构地区：[1]华中科技大学同济医学院法医学系

出　　处：《法医学杂志》2004年第2期77-80,i010,共5页Journal of Forensic Medicine

基　　金：中国博士后科学基金资助项目(2003034459);湖北省自然科学基金资助项目(2003ABA140)

摘　　要：目的利用基因芯片技术,研究人挫伤脑组织中基因表达差异。方法从脑组织提取mRNA,通过OTS-2.2S基因芯片,研究脑挫伤组织与对照脑组织细胞原癌及抑癌相关基因特异性表达差异。结果发现在挫伤脑组织中,HoJ-1和KIAAOO65基因表达水平显著下降,而p107mRNA表达水平显著升高。结论在脑挫伤中仅发现3条基因的表达有显著性差异;本研究是对脑损伤后基因表达水平及其法医学意义进行的探索性研究。Objective To screen the differential expression of oncogenes and tumors suppressed genes(OTS genes) after human brain contusion by cDNA microrarray. Mathods The total RNAs isolated from normal and contusion human brain tissues were purified by Oligotex to obtain mRNAs. Both sources of mRNAs were reversely transcribed to cDNAs with the incorporation of fluorescent dUTP to prepare the hybridization probes. The probe from normal tissue and the contusion brain tissue were labeled with Cy3-dUTP and Cy5-dUTP respectively. The mixed probes were hybridized to the BioDoor Chip OTS-2.2S, a cDNA microarray which contains 227 oncogenes and tumors suppressed genes. After high-stringent washing, the cDNA microarray was scanned for the fluorescent signals and showed differences between two tissues. Results Among the 227 target genes, 3 genes including Human carcinoma associated HOJ-1(HoJ-1), Human KIAAOO65 gene,Human retinoblastoma related protein (p107) gene, showed distinct deference in expression level between the human brain contusion tissue and normal tissue. Conclusion The 3 genes in the brain contusion was significantly the differential expression by OTS 2.2S cDNA microarray. Further analysis of these genes will be helpful to understand the molecular mechanism of brain injury and utilization in forensic medicine.

关 键 词：OTS-2.2S基因芯片 脑挫伤 基因表达 脑组织 抑癌基因 原癌基因 

分 类 号：R651.15[医药卫生—外科学]