乙型肝炎病毒基本核心启动子T^(1762)A^(1764)联合突变的临床意义  

The Clinical Significance of the Basic Core Promoter T^(1762)A^(1764)Mutation of Hepatitis B Virus

作　　者：徐萍[1] 吴建美[1] 钱绩虎[1] 

机构地区：[1]南通医学院微生物学教研室,南通226001

出　　处：《南通医学院学报》2004年第2期133-135,137,共4页ACTA Academiae Medicinae Nantong

摘　　要：目的 :研究乙型肝炎患者乙型肝炎病毒 (HBV)基本核心启动子 (BCP) T1 76 2 A1 76 4联合突变情况及其临床意义。方法 :采用聚合酶链反应 (PCR)与限制性长度片段多态性分析 (RFL P)相结合 ,检测 130例乙型肝炎患者 BCP区核苷酸 (nt) 176 2碱基 A→ T和 176 4碱基 G→ A联合突变。结果 :HBe Ag(+)和 HBe Ab(+)两组患者中 BCP T1 76 2A1 76 4联合突变率的不同频率分别为慢性乙型肝炎患者 (Chronic hepatitis B,CHB) 4 0 .0 %和 85 .7% ,无症状携带者(Asym ptom atic carrier,ASC) 2 2 .2 %和 6 5 .0 % ,献血员为 5 %。BCP T1 76 2 A1 76 4双点联合突变组 HBV- DNA≥ 10 5cps/ml检出例数 81例 (96 .4 % ) ,非突变组 HBV- DNA≥ 10 5cps/ml检出例数 2 5例 (5 4 .3% )。结论 :BCP T1 76 2 A1 76 4联合突变与乙型肝炎的发生、发展以及预后有关 ,可能是 CHB与 ASC患者 HBe Ag(- )的原因之一 ,可促进乙肝病毒繁殖。对慢性乙型肝炎患者检测 HBV BCP T1 76 2 A1 76Objective:To study the T 1762A 1764 mutation in the basic core promoter (BCP) region of hepatitis B (HBV) in Nantong viral hepatitis B patients. Methods:The BCP T 1762A 1764 mutations were detected by combining polymerase chain reaction (PCR) with restriction fragment length polymorphism (RFLP). Results: The BCP T 1762A 1764 mutation frequency in two groups HBeAg (+) and HBeAb (+) are 40.0% and 85.7% in CHB,22.2% and 65.0% in ASC, and 5% in donors.81(96.4%)in BCP mutant group and 25 (54.3%)in non-BCP mutant group were observed above 10 5 cps/ml in HBV DNA contents.Conclusions:There is a significant difference of BCP T 1762A 1764 mutation between patients and blood donors, which suggests that the BCP T 1762A 1764 mutation be one of the major causes for HBV infection in CHB and ASC patients with HBeAg (-), and that the mutation be revelant to the development, chronicity and fulmination of hepatitis B; A significant difference in HBV DNA content is also found between the T 1762A 1764 mutation group and non-mutation group,and the result suggests that the T 1762A 1764 mutation may accelerate the replication of HBV.

关 键 词：乙型肝炎病毒 基本核心启动子 变异 限制性片段长度多态性分析 

分 类 号：R512.6[医药卫生—内科学]