乙醇预处理诱导增强肝脏对缺血再灌流的耐受性  

Tolerance enhancement on the liver ischemia-reperfusion injuries in rats by ethanol pretreatment

作　　者：冯晓彬[1] 郑树国[1] 李昆[1] 朱瑾[1] 董家鸿[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,中国人民解放军西南肝胆外科医院,重庆400038

出　　处：《中华肝脏病杂志》2004年第7期420-423,共4页Chinese Journal of Hepatology

基　　金：国家自然科学基金(39970727);国家自然科学基金海外青年合作研究项目(30228030)

摘　　要：目的 探讨胃饲乙醇预处理对肝脏缺血再灌流损伤的影响,确定适当预处理方案,并作初步评价。方法 雄性成年Wistar大鼠36只,随机分6组,胃饲乙醇浓度40％,A组8g/kg、B组7g/kg、C组6g/kg、D组5g/kg、E组4g/kg、正常组0g/kg;以中毒症状及肝组织病理为指标,判定大鼠急性乙醇胃饲中毒剂量;选定剂量范围继续本实验。大鼠78只,随机分为4组:正常对照组、胃饲乙醇组、缺血组(IR)、胃饲乙醇预处理组(EP);采用尾叶转流下的肝缺血模型,于再灌流3、6、12、24h留取标本;采用正交设计,以乙醇浓度、剂量、胃饲时机为因素,分设3个水平,大鼠54只,肝缺血90min,于再灌流24h采样检测。结果 急性胃饲乙醇≤5g/kg预处理后,动物中毒症状轻,无死亡;乙醇预处理可以在一定程度上减轻肝脏90 min的缺血再灌流损伤;在A1B1C3预处理模式下,即40％乙醇5g/kg胃饲后24 h行肝缺血手术对大鼠肝脏缺血再灌流损伤的保护作用最强。结论 适当剂量的乙醇胃饲预处理是一种安全的预处理措施,有望成为增强肝脏对缺血再灌流损伤耐受性的一种较好的预处理方式。Objective To investigate the feasibility and efficacy of the ethanol precreatment. Study was designed to confirm the proper range of the ethanol according to the toxicity and mortality, and then evaluate the possibility of application of the ethanol pretreatment. Methods (1)Thirty six male adult wistar rats pretreated with 40% ethanol were divided randomizely into six groups by different dosage: group A(8g/kg), group B(7g/kg), group C(6 g/kg), group D(5g/kg), group E(4g/kg), normal control group(0 g/kg). The safe dosage range of ethanol in rats was predicfed by the observation of the symptoms after ethanol administration and pathological changes after 24 h. (2) Based on the results of experiment(1), this experiment were set as follows: 78 wistar rats were divided randomizely into 4 groups: normal control group, ethanol group, ischemia/reperfusion group(IR), ethanol pretreatment group(EP), in each group, the specimen were harvested from the rats at 3, 6, 12, 24 h after reperfusion and then were determined by different methods. (3) Based on the three variant factors(concentration, dosage and proper time for ethanol pretreatment), a orthogonal test were designed to optimize the ethanol pretreatment. 54 wistar rats used in this step were all subjected to hepatic schema procedure for 90 min and the specimens were harvested at 24 h after reperfusion. Results Less than 5g/kg ethanol is safe to the rat, and it can reduce the 90 minutes IR injuries to the liver. Under the mode of A1B1C3, the more protection can be got for hepatic ischemia/reperfusion injuries. Conclusions Proper dose of ethanol gavages to the rat is a safe pretreatment method, it maybe enhance the tolerance of rat liver to the I/R injuries.

关 键 词：乙醇预处理 肝脏缺血 再灌流损伤 耐受性 

分 类 号：R657.3[医药卫生—外科学]