甲醛致核酸损伤作用的实验研究  

Experimental study of the DNA damage induced by formaldehyde

作　　者：袭著革[1] 晁福寰[1] 杨丹凤[1] 孙咏梅[1] 李官贤[1] 张华山[1] 张伟[1] 杨玉花[1] 刘焕亮[1] 

机构地区：[1]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《环境科学学报》2004年第4期719-722,共4页Acta Scientiae Circumstantiae

基　　金：国家科技部"十五"攻关项目(2001BA704B01);国家科技部社会公益基金(2001DIA1001)

摘　　要：应用单细胞凝胶电泳技术(SCGE)和液相色谱-电化学检测法(HPLC-EC)研究了典型环境污染物甲醛对DNA分子的损伤效应.结果表明:甲醛不仅可诱导人外周血淋巴细胞DNA发生链断裂,还可引起DNA DNA,DNA 蛋白质交联;甲醛与小牛胸腺DNA的体外作用较弱,但在铁离子介导下对DNA的氧化能力增强,可产生一定量的8 羟基脱氧鸟苷(8 OHdG)加合物;动物实验表明甲醛诱导大鼠肺组织DNA氧化损伤生成8 OHdG,提示甲醛较强的遗传毒性.The DNA damages induced by the typical environmental pollutant formaldehyde was studied with single cell gel electrophoresis(SCGE) and high performance liquid chromatography with electrochemical detection(HPLCEC). The results showed that formaldehyde could not only cause DNA strand breakage but also DNADNA, DNAprotein crosslinks of lymphocytes of human peripheral blood. The reaction of formaldehyde with DNA in vitro is weak, but the oxidative ability is enhanced and the reaction could produce a number of 8OHdG adducts mediated by the Fe2+. The animal experiment showed that formaldehyde could cause the oxidative DNA damage of rat lung tissues, which suggested that formaldehyde have the stronger genotoxicity.

关 键 词：甲醛 环境致癌物 DNA 蛋白质 8-羟基脱氧鸟苷 单细胞凝胶电泳技术 

分 类 号：X503[环境科学与工程—环境工程] X131