丹参对大鼠缺血再灌注肝细胞凋亡作用的研究  

Effects of salivae miltiorrhizae on apoptosis of hepatic cells in ischemia - reperfusion injury in rats

作　　者：李素芝[1] 孙克勤[1] 刘厚东[1] 王茂旭[1] 杜富波[1] 王福永[1] 

机构地区：[1]西藏军区总医院,西藏拉萨850003

出　　处：《西南国防医药》2004年第4期349-350,共2页Medical Journal of National Defending Forces in Southwest China

摘　　要：目的 :研究丹参对缺血再灌注 (IR)后肝细胞凋亡的作用。方法 :用TUNEL法观察IR后的肝细胞凋亡 ,并用免疫组织化学法检测相关基因表达变化 ;以丹参注射液干预IR的大鼠模型 ,观察丹参对IR后肝细胞凋亡的作用。结果 :IR后肝细胞有典型的凋亡特征 ,与丹参组比较肝细胞的凋亡率有显著差异 (P <0 0 1)。IR组Bcl 2表达下调 ,而丹参组的表达量显著增加 (P <0 0 1)。结论 :丹参对IR后的肝细胞凋亡有明显的抑制作用。Objective:To study the effects of salivae miltiorrhizae(SM) on apoptosis of hepatic cells in ischemia - reperfusion(IR) injury in rats. Methods: The apoptosis of hepatic cells of rats with ischemia - reperfusion injury was observed by terminal deoxynuleotidy transferase - mediated nicked labeling(TUNEL) and the expression of apoptosis relative genes was determined by immunohistochemical staining. Then the effects of SM injection on apoptosis of hepatic cells of the animals were investigated. Results: After the ischemia - reperfusion injury, the typical apoptotic morphological characters in hepatic cells of these rats were observed, and there was very significantly difference of the rate of apoptotic liver cells between the IR group and SM group(p<0.01). The expression of bcl - 2 decreased significantly in IR group, but increased in SM group(p<0.01). Conclusion: SM may inhibit the apoptosis of hepatic cells induced by ischemia - reperfusion injury in rats.

关 键 词：丹参 大鼠 肝缺血 再灌注损伤 肝细胞凋亡 治疗 

分 类 号：R259.75[医药卫生—中西医结合]