中药肝力克对肝纤维化大鼠肝组织iNOSmRNA表达的影响  

Effects of Ganlike on the mRNA Expression of iNOS in Rats with Experimental Hepatic Fibrosis

作　　者：夏亮[1] 陈芝芸[2] 严茂祥[2] 项柏康[2] 

机构地区：[1]浙江省立同德医院消化科,浙江杭州310006 [2]浙江省中医院消化研究室

出　　处：《中西医结合肝病杂志》2004年第4期219-221,共3页Chinese Journal of Integrated Traditional and Western Medicine on Liver Diseases

基　　金：浙江省教育厅资助项目(No19990360);浙江省中医管理局资助项目(No2000Y03)

摘　　要：目的 :观察中药肝力克对肝纤维化大鼠肝组织iNOSmRNA (诱生型一氧化氮合酶mRNA)表达的影响 ,探讨其作用机制。方法 :用 40 %CCl4皮下注射建立大鼠肝纤维化模型。造模结束后 ,给予不同剂量的肝力克和秋水仙碱灌胃治疗 9周。采用原位杂交法观察大鼠肝组织中iNOSmRNA的表达情况。结果 :肝纤维化模型组大鼠肝组织iNOSmRNA阳性表达较正常对照组明显升高 (P <0 0 1) ;应用肝力克能明显改善大鼠肝组织炎症活动度和纤维程度 ,能明显下调肝纤维化大鼠肝组织iNOSmRNA表达。结论Objective:To investigate the mRNA expression of the inducible nitric oxide synthase and the effects of Ganlike in rats with experimental liver fibrosis induced by carbon tetrachloride. Methods:SD rats′model of fibrosis was induced by injecting CCl 4. After model being built, they were protected with Ganlike and Colchicine, and normal saline were taken as the control. The expression of iNOS mRNA in all liver tissues was detected by in situ hybridization method. The severity of inflammation and fibrosis in all liver tissuses were measured by semiquantitative scoring. Results: the expression of iNOS mRNA in the hepatic fibrosis were significantly higher than the control group(P<0.01), after the treatment with Ganlike, The severity of inflammation and fibrosis were obviously improved, and the expression of iNOS mRNA in hepatic fibrosis was downregulated. Conclusion:Ganlike can downregulate the expression of iNOS mRNA in hepatic fibrosis, which maybe a possible mechanism of its anti-hepatic fibrosis.

关 键 词：中药 肝力克 肝纤维化 大鼠 肝组织 INOSMRNA 表达 

分 类 号：R285[医药卫生—中药学]