佐剂性关节炎早期病变的X线位相对比成像研究  

In vivo evaluation of early disease progression by X-ray phase contrast imaging in the adjuvant arthritis rat

作　　者：熊壮[1] 余永强[1] 吕益忠[1] 钱银锋[1] 岳莉[1] 蒋诗平[2] 陈阳[2] 田玉莲[3] 

机构地区：[1]安徽医科大学附属医院放射科,合肥230022 [2]中国科学技术大学同步辐射实验室 [3]中国科学院高能物理研究所同步辐射实验室

出　　处：《中华风湿病学杂志》2004年第8期471-475,共5页Chinese Journal of Rheumatology

摘　　要：目的应用X线位相对比成像活体研究佐剂性关节炎(AA)大鼠早期的骨关节改变。方法SD大鼠左后足爪皮下注射弗氏完全佐剂(CFA)建立AA模型后,于病变早期进行连贯的右后足爪X线位相对比成像,观察其骨关节的动态变化,同时进行足爪肿胀度、体重等生理指标的检测和传统X线成像。最后,苏木素-伊红(HE)染色行AA大鼠足爪的病理检查。结果佐剂性关节炎大鼠右后足爪跖趾关节在致炎后第10天,骨质改变不明显,仅表现为周围软组织肿胀。第12天骨性关节面及其下出现骨质破坏、骨质疏松,周围软组织肿胀加重。第14天骨质破坏区扩大、融合,尤其边缘部位表现明显。第16天,骨性关节面及其下骨质破坏区仍然存在,关节间隙与正常间隙比较显示增宽,周围软组织肿胀显著。第18天,可见明确的骨膜新生骨形成,骨质破坏区缩小,关节周围骨质密度增高,软组织肿胀减轻。X线位相对比成像能清楚显示上述改变,而传统X线成像却不能分辨。足爪容积及体重在实验期间出现相应变化,并且与正常组比较差异有显著性。随后的病理检查证实了佐剂性关节炎的存在。结论佐剂性关节炎早期除显著的周围软组织肿胀、关节间隙增宽外,还可出现骨质疏松、骨质破坏以及骨膜增生,并且呈动态变化过程。X线位相对比成像能清楚地活体显示佐剂性关节炎的细微骨质改变,这是?Objective To non-invasively study the early change of bone matrix and soft tissue around inflammed joints in adjuvant arthritis (AA) rat by X-ray phase contrast imaging (XPCI), a new imaging method. Methods AA was established in male SD rats following subcutaneous injection of complete Freund′s adjuvant (CFA) in the left hindpaw. In vivo XPCI evaluation of the early soft tissue and bone changes in AA rats was consecutively performed and compared with changes in right hindpaw size and body weight. Conventional radiography of the right hindpaw was taken at the same time. Finally, AA rats were histologically examined. Results By day 10 after subcutaneous injection of CFA, bone changes in the right hindpaw were not obvious, but swelling of soft tissue appeared. By day 12, bone erosions in articular facet and subarticular-facet, along with osteoporosis, were demonstrated, and swelling of soft tissue was aggravated. By day 14, bone erosions became fused and expanded, especially around margin area of the joint. By day 16, bone erosions still existed, joint space wider than normal, and swelling of soft tissue was significant. By day 18, periosteal new bone formation was seen definitely, destruction of bone decreased, bone density around articular increased, and the swelling of soft tissue subsided. XPCI could clearly distinguish all of these changes that couldn′t be demonstrated by conventional radiography. During the study period, the right hindpaw size and body weight of AA rat also changed greatly compared with normal rat. Histological examination proved that AA had occurred in all rats of adjuvant group. Conclusion Osteoporosis, bone erosion and periosteal new bone formation take place at the early stage of AA and develop dynamically. XPCI can evaluate in vivo these subtle bone changes that are 'blind area' by previous imaging methods, so this new technique can be used as an effective tool in relevant studies.

关 键 词：佐剂性关节炎 早期 X线位相对比成像 骨关节改变 诊断 类风湿关节炎 

分 类 号：R593.22[医药卫生—内科学]