隐性类风湿因子的测定和临床意义的初步探讨  

The study of hidden rheumatoid factor detection and its clinical significance

作　　者：潘丽恩[1] 马骥良[1] 李新民[2] 

机构地区：[1]首都医科大学附属北京友谊医院风湿内科,100050 [2]首都医科大学附属北京友谊医院实验中心,100050

出　　处：《中华风湿病学杂志》2004年第8期485-487,共3页Chinese Journal of Rheumatology

摘　　要：目的研究隐性类风湿因子(HRF)的测定方法,探讨其临床意义。方法用沉淀剂沉淀包含HRF的免疫复合物,解离后再用中和抗体清除变性IgG,最后用酶联免疫吸附试验(ELISA)法测定IgM型的HRF,同时分析其与临床活动性的相关关系。结果用该方法和离子交换柱法同时测定成人活动期类风湿关节炎(RA)患者血清中的HRF水平,二者差异无显著性(P>0.05)。该法与空白对照组比较,其HRF水平高于空白对照组(P>0.05)。用该法测定28例强直性脊柱炎患者血清中的HRF,均未测及。同时发现用该法测定的HRF水平与C反应蛋白(CRP)有一定的相关性(r=0.331,P<0.05),且其HRF的阳性率活动期RA高于非活动期RA(P<0.01)。结论沉淀中和法测定HRF简单、可行、特异,其水平与RA的临床活动有一定的相关性。Objective To study the detection method of hidden rheumatoid factor (HRF) and its clinical significance in adult active rheumatoid arthritis patients. Methods The immuno-complexs which contained HRF were deposited by sediment agents, then HRF was seperated from the complex and detected by ELISA. The relationship between HRF and clinical activity was analysed. Results The positive ratio of HRF had no significant difference between the depositing methods and ion-exchange methods (P>0.05).There was no difference in HRF serum levels between the depositing group and control group (P>0.05). HRF couldn′t be detected in 28 ankylosing spondylitis patients. The correlation was found between the serum levels of HRF and CRP in active RA patients (r=0.331,P<0.05). The HRF rate in active RA patients was higher than that in non-active RA patients(P<0.01). Conclusion Depositing is a simple and specific method for HRF detection. The HRF is associated with disease activity in adult active rheumatoid arthritis.

关 键 词：隐性类风湿因子 测定 类风湿关节炎 酶联免疫吸附试验 

分 类 号：R593.22[医药卫生—内科学]