ApoE^(-/-)训练鼠血总胆固醇升高没有使动脉粥样硬化斑块增长  

Increased Plasma Total Cholesterol did not Promote Development of Atheroma Plaque in ApoE -/- Mice

作　　者：李晖[1] 张宝慧[1] 周兰兰[1] 李可基[1] 

机构地区：[1]北京大学第三医院运动医学研究所,北京100083

出　　处：《中国运动医学杂志》2004年第4期381-385,共5页Chinese Journal of Sports Medicine

摘　　要：目的 :在小鼠模型中 ,探讨有氧运动及其引起的血脂、T淋巴细胞亚群比例和自由基代谢改变对动脉粥样硬化斑块生长的影响。方法 :ApoE基因敲除鼠和C5 7BL/ 6J对照鼠进行不负重游泳 ,12 0min/d ,6d/w ,持续 12w。结果 :安静状态下与对照鼠比较 ,ApoE- /- 鼠血浆TC( 1 91± 0 35mmol/Lvs 14 6± 1 4 6mmol/L ,P <0 .0 0 1)、TG( 0 .96± 0 .30mmol/Lvs 1.2 8± 0 .34mmol/L ,P =0 0 13)升高 ,主动脉壁产生动脉粥样硬化复合斑块。运动后 ,ApoE- /- 鼠TC进一步升高 ( 14 6±1 4 6mmol/Lvs 17 8± 2 96mmol/L ,P =0 0 2 3) ,动脉粥样硬化斑块面积 ( 2 5 4± 7.2vs 2 5 .9± 12 .6,P =0 93)没有显著变化 ,血管壁MDA增加 ( 14 8 8± 16 8mmol/Lvs 2 0 1 3± 38 0mmol/L ,P =0 0 2 0 )。结论 :12周耐力运动后ApoE- /- 鼠血浆TC和血管壁MDA水平升高 ,但TC的升高没有促进动脉粥样硬化斑块的发展。Objective To approach the effects of aerobic exercise on relative changes in T cell subpopulation, free-radical metabolism and development of atheroma plaque. Methods ApoE-deficient mice and homogeneous C57BL/6J control mice participated in a swimming program for 12weeks (120m/d, 6d/w). Results Comparing the results in sedentary groups,ApoE -/- mice developed complex atheroma plaques along aorta walls and showed higher levels of TC(1.91±0.35 mmol/L vs 14.6±1.46 mmol/L,P<0.001),TG(0.96±0.30 mmol/L vs 1.28±0.34 mmol/L,P=0.013). After exercise,ApoE -/- mice had further increased TC levels(14.6±1.46 mmol/L vs 17.8±2.96 mmol/L,P=0.023)in plasma and showed higher MDA levels(148.8±16.8 mmol/L vs 201.3±38.0 mmol/L,P=0.020)in vessel walls. Conclusion After 12w endurance training, the higher levels of TC in plasma and MDA in vessele wall in ApoE -/- mice did not promote atheroma plaque development.

关 键 词：ApoE敲除鼠 血总胆固醇 动脉粥样硬化斑块 T淋巴细胞亚群 自由基代谢 

分 类 号：R87[医药卫生—运动医学]