血管内皮细胞生长因子基因治疗对骨髓微血管照射损伤的修复  

Repairing of TBI-induced damage and remodeling of bone marrow-microvasculature by adenovirus mediated hVEGF_(165) gene therapy in post-BMT mice

作　　者：仲照东[1] 邹萍[1] 游泳[1] 刘凌波[1] 胡中波[1] 郭荣[1] 黄士昂 

机构地区：[1]华中科技大学同济医学院附属协和医院血液科,武汉430022 [2]湖北省干细胞应用与研究中心

出　　处：《中华放射医学与防护杂志》2004年第4期316-318,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：国家自然科学基金海外青年学者基金资助项目 (3 992 80 10 )

摘　　要：目的　探讨内皮生长因子对骨髓移植小鼠骨髓微血管损伤修复的影响。方法　利用腺病毒介导人血管内皮细胞生长因子1 6 5(hVEGF1 6 5)在骨髓移植小鼠体内表达 ,检测骨髓微血管系统和造血细胞容量变化。结果　 +10d接受hVEGF1 6 5基因治疗的小鼠胫骨中央静脉肿胀程度明显轻于注射腺病毒AdEGFP组和常规移植组 (P <0 0 1) ;+2 0d时该组小鼠胫骨中央静脉直径、微血管灌注面积已恢复正常 (P >0 0 5 ) ,且骨髓细胞容量高于其他组 (P <0 0 5 )。结论　VEGF基因体内高效表达可以加快全身照射引起的骨髓移植小鼠骨髓微血管损伤的修复 ,重塑微血管结构 ,促进骨髓造血功能的重建。Objective To explore the effect of vascular endothelial growth factor on the repairing of TBI- induced damage of bone marrow microvasculature in post-BMT mouse. Methods Mediated by adenovirus,hVEGF_ 165 was expressed in TBI -conditioned BALB/c mice with syngeneic BMT. On +5 d,+10 d,+20 d,+30 d,the mice were injected with Chinese ink,following which the transparent tibias or plastic sections were used for analysis. Results hVEGF_ 165 was successfully expressed by Ad-EGFP/hVEGF_ 165 . On +20 d,the diameter of central sinus in the VEGF group restored normally ( P >0.05) and the percentage of microvasculature surface area returned to normal level ( P >0.05). The percent cellularity of BM in the VEGF group began to be higher than that of other groups after +20 d ( P <0.05) . Conclusion The expression of hVEGF_ 165 promotes the repairing of TBI -induced damage and the remodeling of bone marrow microvasculature,the resulting in rapid hematopoietic reconstruction.

关 键 词：血管内皮细胞生长因子 基因治疗 骨髓微血管 照射损伤 全身照射 

分 类 号：R456[医药卫生—治疗学]