低剂量γ射线全身照射对猴免疫功能的影响  

The influence of low-dose total body γ-irradiation on immune function of monkey

作　　者：黎福祥[1] 陈龙[1] 董永英[1] 黄江琼[1] 唐东平[1] 朱波[1] 魏党[1] 唐凯[1] 谢莉萍 陈铭忠[1] 

机构地区：[1]广西医科大学附属肿瘤医院,南宁530021 [2]广西营林开发中心实验动物基地

出　　处：《中华放射医学与防护杂志》2004年第4期330-332,共3页Chinese Journal of Radiological Medicine and Protection

基　　金：广西壮族自治区卫生厅基金资助项目 (9612 )

摘　　要：目的　探索大动物低剂量全身照射的适宜剂量 ,以便评价低剂量全身照射应用于临床的可能性。方法　食蟹猴 32只随机分为 4组 ,用γ射线 (吸收剂量率为 0 175mGy min)分别照射0、30、5 0和 75mGy。分别在环磷酰胺处理前、后 ,亦即照射前和照射后 1、2、8、33和 5 0周检测淋巴细胞亚群、免疫球蛋白及自然肿瘤红细胞花环试验 (NTERT)。结果　结果显示白细胞释放细胞因子尤其是CD1 6 参与免疫调控 ,出现时间 剂量的反应 ;体液免疫中以IgA升高为特征 ,照射后第 8周 5 0和75mGy 2个剂量组与对照组相比差异有显著性 (P <0 0 5 ) ;NTERT结果显示 ,在照射后第 2周 ,第 1、2、3组分别与第 4组相比差异有显著性 (P <0 0 5 ) ,但第 1组分别与第 2、3组相比差异无显著性。结论　γ射线单次低剂量全身照射对免疫功能低下模型的食蟹猴红、白细胞免疫功能有增强其免疫功能的作用。Objective To investigate appropriate the dosage of low-dose total body irradiation(TBI) on large animals in order to evaluate the possibility of clinical therapy. Methods A total of 32 cynomolgus were randomized into four groups which were irradiated with 0,30,50 and 75 mGy γ-rays respectively,at the dose rate of 0.175 mGy/min to observe their lymphocyte sub-populations ,immunoglobulin and the results of natural tumor erythrocyte rosette test(NTERT) before and after receiving cyclophosphamide(CP),namely before and 1,2,8,33 and 50 weeks after irradiation . Results The results indicated that leucocyte release factors,especially CD_ 16 (NK cell), took part in immune regulation and presented a time-dose response. Increase of IgA of the humoral immunity is characteristic,for significant differences were seen between the control group and 75 mGy,50 mGy groups after 8 weeks' irradiation . Meanwhile ,there were significant differences in NTERT result of number 1,2 and 3 groups as compared with that of number 4 group after 2 weeks' irradiation ,but there was no difference between number 1 group and number 2,3 groups. Conclusion These observation suggest that TBI with low-dose γ-rays can increase erythrocyte and leucocyte function in the reduced immune function model of cynomolgus,and the present observation might provide a theoretic basis for low dose TBI clinical therapy.

关 键 词：低剂量 Γ射线 全身照射 猴 免疫功能 体液免疫 

分 类 号：R392[医药卫生—免疫学]