雌二醇对溴氰菊酯染毒大鼠皮层突触体神经毒性的影响  

Effects of estradiol on deltamethrin-induced neurotoxicity in rat cerebro-cortical synaptosomes

作　　者：陈亮[1] 石年[1] 董杰[1] 李涛[1] 陈丹[1] 

机构地区：[1]华中科技大学同济医学院卫生毒理学系,武汉430030

出　　处：《中华劳动卫生职业病杂志》2004年第1期22-25,共4页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金资助项目 ( 30 371 2 2 5)

摘　　要：目的　观察雌激素对溴氰菊酯染毒动物皮层突触体膜兴奋性氨基酸递质释放和ATPase活力的影响。方法　用高效液相色谱 (HPLC)检测不同水平 17β 雌二醇 (10 - 5 、10 - 8、10 - 11mol/L)对 2× 10 - 5 mol/L溴氰菊酯染毒的去卵巢大鼠皮层突触体氨基酸释放的影响 ;用化学比色法检测 17β 雌二醇 (10 - 5 、10 - 8、10 - 11mol/L)对 2× 10 - 4mol/L溴氰菊酯染毒的去卵巢大鼠皮层突触体Na+ K+ ATPase、Mg2 + ATPase、Ca2 + ATPase、Ca2 + Mg2 + ATPase活力的影响 ,并观察雌激素受体拮抗剂Tamoxifen对雌二醇作用的影响。结果　 2× 10 - 5 mol/L溴氰菊酯处理可增加高钾去极化状态下大鼠皮层突触体天冬氨酸 (Asp)和谷氨酸(Glu)的释放 ;10 - 8、10 - 11mol/L的 17β 雌二醇可抑制溴氰菊酯所致高钾去极化状态下突触体Asp和Glu的释放 ,对Asp释放抑制率为 2 8.42 %、2 4.3 6% ,对Glu释放抑制率为 2 1.52 %、14 .57% ;Tamoxifen对 17β 雌二醇的抑制作用未见拮抗。 2× 10 - 4mol/L溴氰菊酯可抑制突触体 4种ATPase活力 ;10 - 5 mol/L雌二醇可拮抗溴氰菊酯对 4种ATPase活力的抑制 ;10 - 8、10 - 11mol/L雌二醇可增加Ca2 + ATPase活力 ;Tamoxifen对雌二醇的拮抗作用仍未见明显影响。结论　 17β 雌二醇对溴氰?ObjectiveTo investigate the neuroprotectiv e effect of estradiol on the release of excitatory amino acid(EAAs) mediator,and the activity of ATPase in cerebro-cortical synaptosome membrane of rats expose d to deltamethrin. MethodsUsing HPLC to detect EAAs rele ase,and colorimeter method to measure the activities of Na +-K +-ATPase,Mg 2+ -ATPase,Ca 2+ -ATPase,Ca 2+ -Mg 2+ -ATPase in the cerebro -cortical synaptosomes of ovariectomized rats exposed to deltamethrin (2×10 -5 mol/L),and treated with different doses of 17β estradiol(10 -5 ,10 -8 ,10 -11 mol/L).Meanwhile,the estrogen receptor(ER) antagonist,tamoxif en,was used to investigate the effect on estradiol. ResultsThe release of Asp and Glu from the cerebro-cortical synaptosomes was signif icantly increased by 2×10 -5 mol/L deltamethrin exposure at the depolarizin g state evoked by 50 mmol/L KCl,while 10 -8 ,10 -11 mol/L 17β estradio l could partly inhibit the effect of deltamethrin on the release of Asp(28.42%, 24.36% ,respectively),Glu(21.52%,14.57%,respectively).The activities of 4 kin ds of ATPase were inhibited by 2×10 -4 mol/L deltamethrin,and these effects could be blocked by 10 -5 mol/L estradiol,while the activity of Ca 2+ -ATPase was increased by 10 -8 ,10 -11 mol/L of estradiol.However,no o bvious antagonistic effect of tamoxifen on the function of estradial on EAAs rel ease or the activities of ATPase was found. ConclusionEs tradiol showed certain neuroprotective effect on the release of EAAs and the inh ibition on ATPase induced by deltamethrin.The effect of estradiol on synaptosome s may indicate the nongenetic mechanism of estradiol.

关 键 词：雌二醇 溴氰菊酯染毒 大鼠 皮层突触体 神经毒性 兴奋性氨基酸药 

分 类 号：R96[医药卫生—药理学]