过继转输正常妊娠孕鼠的T细胞抑制自然流产孕鼠对胚胎的排斥  

Adoptive transfer of T cells from the normal pregnancy mice suppresses maternal rejection to the allogeneic fetus in the abortion-prone mice

作　　者：金莉萍[1] 李大金[1] 王明雁[1] 朱影[1] 孟毅[1] 袁敏敏[1] 

机构地区：[1]复旦大学附属妇产科研究所生殖免疫研究室,上海200011

出　　处：《中华微生物学和免疫学杂志》2004年第8期632-635,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金 ( 3 9770 773 ) ;复旦大学"985工程"项目 ( 985B3 6) ;计划生育药具国家重点实验室课题 (B2 0 2 1)资助

摘　　要：目的　观察过继转输正常妊娠孕鼠的T、B细胞对小鼠自然流产孕鼠妊娠预后的影响。方法　以♀CBA J×♂BALB c为正常妊娠模型 ,♀CBA J×♂DBA 2为自然流产模型 ,于孕 9d ,采用单向混合淋巴细胞反应分析两组孕鼠脾脏免疫细胞对父系抗原的增殖能力及上清液IL 2分泌水平 ,以研究孕鼠母 胎免疫耐受状态 ;并于孕 14d观察两种模型的胚胎吸收率。然后 ,应用免疫磁珠阴性分选两组孕鼠脾脏T细胞和B细胞 ,并将两种细胞分别转输至孕 4d(着床期 )的♀CBA J×♂DBA 2孕鼠 ,于孕 14d分别观察宿主孕鼠的胚胎吸收率。结果　与自然流产孕鼠比较 ,正常妊娠孕鼠孕 9d脾脏免疫细胞对父系抗原的增殖能力及IL 2的分泌显著下降 (P <0 .0 5 ) ,孕 14d胚胎吸收率也显著下降 (P <0 .0 5 )。与转输自然流产孕鼠的T细胞相比 ,转输正常妊娠孕鼠T细胞的宿主孕鼠的胚胎吸收率显著下降 (P <0 .0 5 ) ;而转输两组B细胞的宿主孕鼠的胚胎吸收率差异无显著性 (P >0 .0 5 )。结论　于孕早期过继转输正常妊娠孕鼠的T细胞能诱导宿主孕鼠母 胎免疫耐受 ,防止母体对胚胎的免疫排斥 ,使自然流产模型的妊娠预后达到正常妊娠水平。ObjectiveTo investigate the effect of the adoptive transfer of T cells and B cells from the normal pregnancy mice on pregnant outcome of the abortion-prone mice. MethodsThe experiments were performed in following two groups: using ♀CBA/J×♂BALB/c matings as the normal pregnancy model and ♀CBA/J×♂DBA/2 matings as the abortion-prone model. We tested the proliferative capacity and IL-2 production by pregnant CBA/J splenocytes in response to paternal splenocytes as stimulator cells in an one-way mixed lymphocyte reaction(MLR) and counted the embryo resorption rate at day 14 of gestation. Thereafter,purified T and B cells were obtained from spleens of the pregnant CBA/J mice using magnetic beads at day 9 of gestation,and then injected intravenously into the pregnant CBA/J mice mated with DBA/2 males at day 4 of gestation (time of implantation). The embryo resorption rate of recipient mice was counted at day 14 of gestation to evaluate the effect of adoptive transfer of T cells from the normal pregnancy mice on pregnant outcome of the abortion-prone mice. ResultsThe embryo resorption rate of the ♀CBA/J×♂BALB/c matings decreased significantly,and in vitro the proliferative index and IL-2 production by pregnant CBA/J splenocytes in response to paternal splenocytes also decreased significantly as compared to that of the ♀CBA/J×♂DBA/2 matings. In the recipient CBA/J mice,these adoptively transferred T cells from the normal pregnancy mice can increase fetal viability,whereas the adoptive transfer of T cells from he abortion-prone mice or B cells from the normal pregnancy mice did not reduce the embryo resorption rate. ConclusionThe adoptive transfer of T cells from the normal pregnancy mice at the early stage of gestation can induce maternal-fetal immuno-tolerance and prevent maternal rejection to the allogeneic fetus,thus leading to the embryo resorption rate of the abortion-prone model to that in normal pregnancy model.

关 键 词：过继转输 妊娠 孕鼠 T细胞抑制 自然流产 胚胎排斥 胚胎吸收率 

分 类 号：R714.21[医药卫生—妇产科学]