痢疾多糖-蛋白结合疫苗小鼠母子抗体传递试验  

The transplacental antibody transfer after maternal immunization with different Shigella conjugate vaccine

作　　者：雍元[1] 杜琳[1] 杜送田[1] 王燕[1] 陈志宏[1] 谢贵林[1] 

机构地区：[1]兰州生物制品研究所第一研究室,730046

出　　处：《中华微生物学和免疫学杂志》2004年第8期658-661,共4页Chinese Journal of Microbiology and Immunology

基　　金：国家创新药物与中药现代化重大项目

摘　　要：目的　以小鼠为试验动物模型 ,用不同的痢疾多糖 蛋白结合疫苗免疫母鼠 ,观察IgG抗体的母婴传递情况。方法　用福氏 2a痢疾多糖 重组铜绿假单胞菌外毒素A(rEPA)结合疫苗 (F2a O SP rEPA)和宋内痢疾多糖 重组铜绿假单胞菌外毒素A结合疫苗 (S O SP rEPA)分别皮下免疫母鼠 ,待其怀孕、分娩 ,用ELISA分别检测母体、子体小鼠的IgG抗体滴度。结果　子体小鼠出生第 2周的IgG抗体水平较高 ,随着小鼠周龄的增加 ,抗体水平逐渐下降。结论　痢疾多糖 蛋白结合疫苗免疫母体后 ,诱导的特异性抗体可经母体传递给子体 ,抗体滴度随时间的推移而逐渐降低。ObjectiveTo study specific IgG transplacental antibody transfer following maternal immunization with different Shigella conjugate vaccine. MethodsMice were subcutaneously immunized with Shigella flexneri 2a conjugate vaccine and Shigella sonnei conjugate vaccine separately,after birth,specific antibody in the mother and baby mice were measured with ELISA. ResultsSpecific IgG antibody reached a high level in filial generation two weeks after birth and gradually droped down. ConclusionAfter immunization with Shigella conjugate vaccine,specific antibody was transferred through placenta from the mother to its babies,and the antibody titer droped down gradually after birth.

关 键 词：痢疾多糖-蛋白 疫苗 小鼠 抗体传递试验 母体免疫 免疫接种 

分 类 号：R392[医药卫生—免疫学]