蟾蜍心肌组织自然冻融的组织学观察  

Histological observation of the toad heart tissue in natural freezing-thawing

作　　者：秦世武[1] 李梦英[1] 李凯霞[2] 李守文 冷黎丽 金鑫[3] 陆金红[4] 

机构地区：[1]佳木斯医学院组胚教研室 [2]佳木斯医学院低温医学研究室 [3]佳木斯医学院电镜室 [4]佳木斯医学院第一附属医院检验科

出　　处：《佳木斯医学院学报》1993年第1期24-25,共2页

摘　　要：本实验以自然温度(-22℃)冻融蟾蜍心脏。冷冻50分钟后,以温水30℃复温,1—2分钟后,蟾蜍复苏。经组织学常规处理,HE染色,光镜观察:结果发现,整体和开胸腹冻融的心肌组织结构完整,心肌纤维、心肌细胞核及闰盘清楚可见。离体冻融的心脏心肌纤维部分断裂和透明玻璃样变,心肌细胞核增大。上述结果表明:在体蟾蜍心脏可以在—22℃冷冻至少50分钟。蟾蜍在30℃温水中复温,1—2分钟后复苏,心肌组织有活力和功能。The toad heart was freezed at natural temprature (--22℃) for 50 minutes and thawed. The toad was recovered after 1 -- 2 minutes. The histological routine management (H. E staine)and olympus microscope observation were adopted. The results suggested that heart histological structure was normal and the muscle fiber, cell nucleus and intercalated disks were clear in toto. In freezing -- thawing heart tissue the muscle fiber was breaked with hya- line degeneration. The cell nuclei was swollen. The results confirm that toad heart could be freezed for 50 minute at --22℃ and thawed at 30℃ and it was recovered after 1 --2 minute and the toad heart had viability and function.

关 键 词：心肌 冻融 组织学 蟾蜍 

分 类 号：R318.52[医药卫生—生物医学工程]