蟾蜍肌组织自然冻融的组织学观察  

Histological observation of the toad muscular tissue after natural freezing—thawing

作　　者：骆茹今 李凯霞[2] 张亚茹[2] 徐康顺 秦世武[2] 李梦英[3] 陆金红[3] 原德新[3] 

机构地区：[1]佳木斯结核医院 [2]佳木斯医学院低温医学研究室 [3]佳木斯医学院组胚教研室

出　　处：《佳木斯医学院学报》1993年第5期20-21,共2页

摘　　要：本实验观察了冬眠状态下,蟾蜍在体和离体的心脏和骨骼肌冻融后形态学变化。Olympus显微镜观察和图像分析:在体冻融蟾蜍心肌和骨骼肌,组织结构完整。肌纤维,肌细胞,毛细血管均清楚可见。结果表明:蟾蜍心脏及骨骼肌可在自然温度—22℃暴露冷冻50分钟。以500ml温术为30℃复温,1—2分钟,使其心脏和骨骼肌组织恢复活力和功能。Histological change of freezing—thawing heart and skeletal muscles.of toad in vivo and vitro was analysed by means of olympus microscope and picture observation. The results suggested that the heart and skeletal muscles intact; the muscle fibers,muscle cells and capillaries were seen clearly. Our results indicated that the activity of toad heart and skeletal muscle might be recovered by freezing for 50 minutes(—22℃)and thawing with 500ml water (+30℃).

关 键 词：蟾蜍 冻融 肌组织 活力 

分 类 号：R645[医药卫生—外科学]