一种从人血凝块中提取基因组DNA的方法  

A Simple Method for DNA Extraction from Human Blood Clot

作　　者：陈立[1] 姜莉[1] 罗阳[1] 张学[1] 

机构地区：[1]中国医科大学医学基因组学研究室卫生部细胞生物学重点实验室,沈阳110001

出　　处：《细胞生物学杂志》2004年第5期548-549,共2页Chinese Journal of Cell Biology

摘　　要：介绍了一种新的从人血凝块中提取基因组DNA的方法,用该方法提取的DNA成功地应用于PCR和限制性酶切等后续实验中。基本过程为首先机械粉碎,然后高盐高EDTA溶液处理,含蛋白酶K和SDS的消化液变换温度消化,苯酚氯仿抽提。The method described here enables to extract DNA from human blood clots. The key procedures included: blood clots were homogenized with 80 g/L NaCl and 10% EDTA, the homogenized samples were lysed with 500 f33l of digestion buffer (10 mmol/L Tris, 1 mmol/L EDTA, 20 g/L SDS, 300 f33g/ml proteinase K) and incubated-for 2 h at 56 f44, then the homogenized samples were incubated overnight at 37 f44. This method was reliable for obtaining high quantities of DNA suited for PCR and restriction digest.

关 键 词：血凝块 人 基因组DNA 聚合酶链反应 

分 类 号：R346[医药卫生—基础医学]