肺微血管内皮细胞的分离及其在单核细胞粘附中的应用  

Isolation Of Pulmonary Microvascular Endothelial Cells and Their Apply in Monocyte Adhesion Assay

作　　者：王海杰[1] 李鸿帅[1] 谭玉珍[1] 

机构地区：[1]复旦大学上海医学院人体解剖与组织胚胎学系,邮政编码上海200032

出　　处：《微循环学杂志》2004年第4期10-12,F003,共4页Chinese Journal of Microcirculation

基　　金：高等学校博士点专项科研基金项目 ( 2 0 0 0 0 2 65 0 42 0 0 3 0 2 460 3 6)

摘　　要：目的 :研究肺微血管内皮细胞的分离和纯化方法 ,探讨内皮细胞的透明质酸对于单核细胞粘附的作用。方法 :用两次植块法分离狗和大鼠的肺微血管内皮细胞。通过粘附实验 ,观察单核细胞在内皮细胞单层上的粘附。结果 :第二次植块 3天后 ,内皮细胞自肺组织块边缘迁出 ,2周后内皮细胞生长形成单层。在扫描电镜下 ,内皮细胞表面有许多微绒毛和小凹。用透明质酸酶消化内皮细胞表面的透明质酸后 ,粘附的单核细胞数显著减少。结论 :两次植块法是一种理想的肺微血管内皮细胞的分离和纯化方法。Objective: To investigate isolation of pulmonary microvascular endothelial cells (PMVECs) and effect of hyaluronan of PMVECs on adhesion of monocytes.Method: PMVECs were isolated from dogs and rats with two-time seeding of the pulmonary tissue. In cell adhesion experiments, adhesion of monocytes on the monolayers of PMVECs was examined.Results: PMVECs migrated from the tissue masses 3 day after the second seeding. PMVECs are grown to confluent monolayers 2 week after the seeding. There are many microvilli and caveolae on PMVECs. Monocytes adhered on the PMVEC monolayers decreased after hyaluronan on PMVECs was digested with hyaluronidase.Conclusion: The two time seeding is a desirable method for isolating PMVECs. Hyaluronan on PMVECs promotes adhesion of monocytes on pulmonary microvascular endothelium.

关 键 词：肺微血管 内皮细胞 分离 单核细胞 

分 类 号：R563[医药卫生—呼吸系统]