改性聚β-羟基丁酸酯大鼠同种异体肾上腺细胞移植的研究  

Transplantation of adrenocortical cells among allorat using PHB Modified by Gelatin as cell carrier

作　　者：程帆[1] 胡平[2] 张孝斌[1] 张杰[1] 刘修恒[1] 李伟[1] 吴天鹏[1] 

机构地区：[1]武汉大学人民医院泌尿外科,430060 [2]清华大学化工科学与技术研究所

出　　处：《中华实验外科杂志》2004年第10期1233-1235,共3页Chinese Journal of Experimental Surgery

基　　金：湖北省自然科学基金资助项目(2 0 0 3ABA1 84)

摘　　要：目的　探讨以聚 β 羟基丁酸酯 (PHB)为细胞载体进行肾上腺细胞移植治疗肾上腺皮质功能不全的可行性。方法　利用PHB作为细胞载体进行肾上腺细胞移植 ,体外观察PHB对培养的大鼠肾上腺细胞增殖、激素分泌功能的影响。将种植了大鼠肾上腺细胞的PHB植入到双侧肾上腺切除的模型大鼠体内 ,观察细胞移植受鼠体内激素水平的动态变化及局部组织学变化。结果　PHB及改性材料明胶对体外培养的肾上腺细胞增殖活性 (1.87± 0 .0 3比 1.91± 0 .0 4,P >0 .0 5 )以及分泌功能均无影响 (3 6.43± 3 .65 )ng/L比(3 1.72± 2 .49)ng/L ,P >0 .0 5 )。术后 8周实验组大鼠体内皮质酮与醛固酮水平比单纯双侧肾上腺切除组的激素水平要高 (P <0 .0 5 )。材料在植入体内 1周后可见较多炎性细胞浸润 ,有大量纤维结蒂组织包绕 ;随着时间的延长 ,炎性细胞逐步减少 ,纤维结缔组织逐步消散 ,材料逐步降解 ,血管长入。Objective To investigate the feasibility of transplantation of adrenocortical cells to therapy insufficiency of adrenocortical function using Poly-β-hybroxybutyric acid (PHB) as cell carrier.Methods Adrenocortical cells were cultured with PHB modified by Gelatin.The effects of PHB and gelatin on adrenocortical cells from rats in the aspects of proliferation and secreting function were evaluated by MTT and RIA methods.After cells were cultured in vitro for about 7 days,they were implanted into rats bearing bilateral adrenalectomy.The changes of blood corticosterone and aldosterone and local histological changes in these rats were observed.Results There were no effects of PHB and gelatin on adrenocortical cells from rats in the aspects of proliferating (1.87± 0.03 vs 1.91± 0.04, P> 0.05) and secreting function (36.43± 3.65 ng/L vs 31.72± 2.49 ng/L, P> 0.05). The blood corticosterone and aldosterone level in study group were higher than in control group (P< 0.05) at 8th week after operation.Histologic study showed inflammatory cells were decreased as time passed,fibrous tissue disappeared step by step,material degraded and blood vessel grow into material.Conclusion PHB has no effects on the survival,proliferation,and secreting function of adrenocortical cells.After adrenocortical cells were transplanted into adrenalectomized rats,they can secrete corticosterone and aldosterone which can satisfy the needs of adrenalectomized rats.PHB can be degraded slowly in vivo.Transplantation of adrenocortical cells using PHB as cell carrier to theat insufficiency of adrenocortical function has clinlical possibility.

关 键 词：改性聚β-羟基丁酸酯 大鼠 同种异体肾上腺 细胞移植 载体 

分 类 号：R699.3[医药卫生—泌尿科学]