肝力克对肝纤维化大鼠肝组织基质金属蛋白酶-2及其抑制物的影响  

EFFECTS OF GANLIKE ON mRNA EXPRESSION OF MMP-2 AND TIMP-2 IN LIVER TISSUES OF RATS WITH HEPATIC FIBROSIS

作　　者：严茂祥[1] 陈芝芸[1] 陈宝珍[1] 项柏康[1] 

机构地区：[1]浙江省中医院,杭州310006

出　　处：《中国中医药科技》2004年第6期327-328,共2页Chinese Journal of Traditional Medical Science and Technology

基　　金：浙江省教育厅基金资助项目No.19990 3 60;浙江省中医药管理局基金资助项目No.2 0 0 0Y0 3

摘　　要：目的 :观察中药肝力克对实验性肝纤维化大鼠基质金属蛋白酶 - 2及其抑制物的影响。方法 :4 0 %CCl4皮下注射 ,建立大鼠肝纤维化模型 ;将肝纤维化大鼠随机分为肝纤维化模型组、肝力克大剂量组、肝力克中剂量组和肝力克小剂量组 ,分别给药。取肝组织常规HE染色 ,观察大鼠肝组织炎症活动度和肝纤维化程度 ,用原位杂交法检测大鼠肝组织中MMP - 2及TIMP - 2mRNA表达。结果 :模型组大鼠肝组织炎症活动度计分及肝纤维化程度计分均较正对照组明显升高 (P <0 0 1) ;经肝力克大、中、小剂量组治疗后 ,大鼠肝组织炎症活动度计分及肝纤维化程度计分均较肝纤维化模型组大鼠明显降低 (P <0 0 5 ,P <0 0 1) ;模型组大鼠肝组织MMP - 2、TIMP - 2mRNA表达均较正常对照明显升高 (P <0 0 1) ,经肝力克治疗后 ,大、中、小剂量组大鼠肝组织TIMP - 2mRNA表达较肝纤维化模型组明显降低 (P <0 0 5 ) ;但MMP - 2mRNA表达与肝纤维化模型组大鼠相比差异无显著性意义 (P >0 0 5 )。结论 :肝力克有明显减轻实验性肝纤维化大鼠肝组织炎症活动度及纤维化程度的作用 ;下调肝纤维化大鼠肝组织TIMP - 2mRNA表达 ,可能是其抗纤维化作用机理之一。Objective:To investigate the effects of ganlike on the mRNA expression of MMP-2 and TIMP-2 in the rats with experimental hepatic fibrosis,and to explore the possible mechanism of ganlike's anti-hepatic-fibrosis.Methods:The hepatic fibrosis model was induced in the experimental rats by subscutaneous injection of 40% CCl 4 After hepatic fibrosis model being built,the rats in the ganlike groups were respectively given ganlike at the different dosages,the rats in the normal control and the hepatic fibrosis model group were given the same volume of saline.At the end of the eighteenth week,all the rats were killed.The severity of inflammation and fibrosis in all rats'liver tissues were measured by semiquantitative scoring.At last,The mRNA expression of MMP-2 and TIMP-2 in liver tissues were detected by in situ hybridization.Results:(1)Compared with the normal control group,the severity of inflammation scoring and hepatic fibrosis scoring in the liver tissues of hepatic fibrosis model group showed significant difference(P<0 01);Compared with the hepatic fibrosis model group,the severity of inflammation scoring and the liver fibrosis scoring in the liver tissues of all the ganlike groups became obviously lighter (2)Compared with the normal control group,the expression of MMP-2 and TIMP-2 mRNA in liver tissues of the hepatic fibrosis model group were obviously higher,which showed significant difference;after the treatment,the expression of TIMP-2 in the different dosages of ganlike were obviously lower,the expression of MMP-2 showed no significant influence(P>0 05).Conclusion ①Ganlkie may reduce the severity of inflammation scoring and hepatic fibrosis.②The mRNA expression of MMP-2、TIMP-2 in the liver tissues of the experimental hepatic fibrosis was enhanced,so MMP-2、TIMP-2 may be improtant factors in treatment of hepatic fibrosis.③Ganlike may downregulate the mRNA expression of TIMP-2 in the liver tissues of the experimental hepatic fibrosis,which may be the possible mechanism of ganlike's anti-hepaticfibro

关 键 词：肝力克 肝纤维化 大鼠 肝组织基质金属蛋白酶-2 抑制物 

分 类 号：R285[医药卫生—中药学]